| Journal: | Acta scientiarum. Biological sciences |
| Database: | PERIÓDICA |
| System number: | 000433609 |
| ISSN: | 1679-9283 |
| Authors: | Limeira, Daniel Meneguello1 Santos, Mateus Henrique2 Mateus, Rogério Pincela3 Almeida, Mara Cristina de2 Artoni, Roberto Ferreira2 |
| Institutions: | 1Universidade Federal de Sao Carlos, Departamento de Genetica e Evolucao, Sao Carlos, Sao Paulo. Brasil 2Universidade Estadual de Ponta Grossa, Departamento de Biologia Estrutural, Molecular e Genetica, Ponta Grossa, Parana. Brasil 3Universidade Estadual do Centro-Oeste, Departamento de Ciencias Biologicas, Guarapuava, Parana. Brasil |
| Year: | 2019 |
| Volumen: | 41 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Analítico, descriptivo |
| English abstract | Access the genetic variability of endangered and isolated populations has become an important conservation tool. Astyanax scabripinnis is a well-known fish model for genetic studies, forming very isolated populations in headwaters. Besides that, this species frequently presents supernumerary chromosomes, which elevates the interest on genetic studies. Genetic diversity of an Astyanax scabripinnispopulation from the Atlantic Forest (Serra da Mantiqueira region, Brazil) was assessed with microsatellite markers for the first time. Since microsatellite markers are not described for this species, we tested markers described for a related species for transferability to A. scabripinnis. Six polymorphic loci were sufficiently reliable for population genetic analysis. We found that this population passed through a recent bottleneck because of the presence of an excess of heterozygotes, low allelic diversity, heterozygosity excess, and small effective population size. Individuals with and without B chromosomes were previously identified in this population and our study found private alleles in the individuals without B chromosomes. Furthermore, when individuals without B chromosomes were removed from the analysis, the population did not present heterozygosity excess, suggesting that the bottleneck event was driven by individuals with B chromosomes. Our results provide an insight into the value of microsatellite markers as molecular tools and is the first genetic study using molecular data of A. scabripinnis from this area |
| Disciplines: | Biología |
| Keyword: | Peces, Genética, Variabilidad genética, Heterocigosidad, Marcadores moleculares, Microsatélites, Indice de fijación, Cromosomas supernumerarios, Astyanax scabripinnis, Characidae |
| Keyword: | Fish, Genetics, Genetic variability, Heterozygosity, Molecular markers, Microsatellites, Fixation index, Supernumerary chromosomes, Astyanax scabripinnis, Characidae |
| Full text: | http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/47323/751375148696 |
