X-ray microtomography in comparison to radiographic analysis of mechanically damaged maize seeds and its effect on seed germination



Document title: X-ray microtomography in comparison to radiographic analysis of mechanically damaged maize seeds and its effect on seed germination
Journal: Acta scientiarum. Agronomy
Database: PERIÓDICA
System number: 000460121
ISSN: 1679-9275
Authors: 1
1
2
2
Institutions: 1Universidade de Sao Paulo, Escola Superior de Agricultura "Luiz de Queiroz", Piracicaba, Sao Paulo. Brasil
2Empresa Brasileira de Pesquisa Agropecuaria, Instrumentacao Agropecuaria, Sao Carlos, Sao Paulo. Brasil
Year:
Volumen: 41
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Experimental, analítico
English abstract Among the most relevant aspects of seed production, mechanical damage may affect seed germination and reduce health and vigor. This study introduces a noninvasive high-resolution imaging procedure for evaluating the mechanical damage to maize seeds and the effects on seed germination. Seeds with different levels of mechanical damage were evaluated using a benchtop micro-computed tomography system (micro-CT) and digital X-ray equipment. The two-dimensional transaxial, coronal and sagittal micro-CT sections were used to inspect the seed anatomy and the mechanical injuries in the internal seed tissue. Germination tests were performed using paper towel rolls (25°C for 7 days) in which the seedling length was evaluated on a daily basis, and the seedling dry biomass was measured at the seventh germination day. The micro-CT cross-sectional images allowed an efficient spatial characterization of the mechanical damage inside the seeds. On average, mechanically damaged seeds produced seedlings with a length 24% shorter and a dry biomass 65% less than that of the undamaged seeds. We concluded that the micro-CT technique provides an efficient means to inspect mechanically damaged maize seeds and allows for a reliable association with germination response
Disciplines: Agrociencias,
Biología
Keyword: Gramíneas,
Anatomía e histología,
Maíz,
Zea mays,
Semillas,
Morfología vegetal,
Tomografía computarizada,
Rayos X-3D
Keyword: Gramineae,
Anatomy and histology,
Corn,
Seeds,
Zea mays,
Morfology,
Seed internal morphology,
3D X-ray imaging,
Computed tomography
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