The culture medium volume and the inoculation method should be considered in semi-quantitative screening of calcium phosphate solubilization by bacteria
Document title: The culture medium volume and the inoculation method should be considered in semi-quantitative screening of calcium phosphate solubilization by bacteria
Journal: Acta scientiarum. Agronomy
Database: PERIÓDICA
System number: 000460036
ISSN: 1679-9275
Authors: 1
1
1
1
Institutions: 1Universidade Federal de Lavras, Departamento de Ciencia do Solo, Lavras, Minas Gerais. Brasil
Year:
Volumen: 42
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Experimental, analítico
English abstract Agar media with insoluble phosphates are used for semi-quantitative screening of phosphate-solubilizing bacteria based on the solubilization halo formed around the colonies. We show that the volume of the culture medium (15, 20, and 30 mL) and the inoculation method (toothpick vs microdrop) strongly influence the diameter of the solubilization halo, and this should be considered in advance selection of the isolates most efficient in this process
Disciplines: Biología
Keyword: Bacterias,
Fosfato de calcio,
Rizobacterias,
Medios de cultivo,
Inoculación
Keyword: Bacteria,
Calcium phosphate,
Rhizobacteria,
Culture media,
Inoculation
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