Physiological analysis and gene expression analysis of ZmDBP3, ZmALDH9, ZmAN13, and ZmDREB2A in maize lines
Document title: Physiological analysis and gene expression analysis of ZmDBP3, ZmALDH9, ZmAN13, and ZmDREB2A in maize lines
Journal: Acta scientiarum. Agronomy
Database: PERIÓDICA
System number: 000460001
ISSN: 1679-9275
Authors: 1
2
2
2
2
2
Institutions: 1Universidade Federal de Lavras, Departamento de Biologia, Lavras, Minas Gerais. Brasil
2Universidade Federal de Lavras, Departamento de Agricultura, Lavras, Minas Gerais. Brasil
Year:
Volumen: 42
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Experimental, analítico
English abstract The objective of this study was to evaluate gene expression related to water deficit tolerance in maize lines. For this, lines previously classified as tolerant (91-T and 32-T) and non-tolerant (24-NT and 57-NT) to water deficit were used. The seeds of the four lines were evaluated for emergence and emergence speed index, and the seedlings were evaluated for root and shoot length under two conditions of water availability (70 and 10% substrate water retention capacity). In transcript analysis, the expression of several genes associated with water deficit tolerance, ZmDBP3, ZmALDH9, ZmAN13, and ZmDREB2A, was evaluated by qRT-PCR for the 91-T and 57-NT lines. It can be concluded that soil water deficiency did not reduce root development. However, the shoot length was significantly lower under dry conditions. Through transcript analysis, the genes ZmDBP3 and ZmAN13 were identified as potential markers for the early selection of maize lines tolerant to water deficit
Disciplines: Agrociencias,
Biología
Keyword: Gramíneas,
Fisiología vegetal,
Genética,
Maíz,
Estrés hídrico,
Zea mays,
QRT-PCR
Keyword: Gramineae,
Plant physiology,
Genetics,
Corn,
Water stress,
Zea mays,
QRT-PCR
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