| Journal: | Acta scientiarum. Agronomy |
| Database: | PERIÓDICA |
| System number: | 000459910 |
| ISSN: | 1679-9275 |
| Authors: | Schneider, Theodoro1 Rizzardi, Mauro Antônio2 Grando, Magali Ferrari2 Ceccon, Cássia Canzi2 Nunes, Anderson Luis3 Didone, Dielli2 |
| Institutions: | 1Universidade de Cruz Alta, Cruz Alta, Rio Grande do Sul. Brasil 2Universidade de Passo Fundo, Passo Fundo, Rio Grande do Sul. Brasil 3Instituto Federal de Educacao Ciencia e Tecnologia, Sertao, Rio Grande do Sul. Brasil |
| Year: | 2021 |
| Volumen: | 43 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, analítico |
| English abstract | The elucidation of the resistance mechanism of weeds to herbicides is important for management practices. The objective of this work was to investigate the resistance mechanism of glyphosate-resistant C. sumatrensis biotypes by determining the expression levels of the constitutive gene epsps and two ABC transport protein-coding genes designated m7 and m11 with RT-qPCR. Two biotypes of C. sumatrensis were evaluated: one resistant and one susceptible to glyphosate. The treatments consisted of the absence or application of two doses of glyphosate (1,080 and 8,640 g a.e. ha-1). Plant leaves were collected at 1 and 4 days after herbicide application. No difference was observed in epsps gene expression between the studied biotypes. The expression of the m7 and m11 genes revealed that both genes had higher relative expression in the resistant biotype with the application of glyphosate at both doses. The overexpression of the m7 and m11 genes in the resistant biotype treated with glyphosate reveals that these genes play a role in herbicide resistance. These genes may be involved in the sequestration of glyphosate into the vacuole lumen in the resistant C. sumatrensis biotype studied |
| Disciplines: | Biología |
| Keyword: | Botánica, Genética, Conyza sumatrensis, EPSPs, Glifosato |
| Keyword: | Botany, Genetics, Conyza sumatrensis, Glyphosate, EPSPs |
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