The intestinal tract as the major source of interleukin 6 production during abdominal aortic clamping and hind limb ischaemia-reperfusion injury



Document title: The intestinal tract as the major source of interleukin 6 production during abdominal aortic clamping and hind limb ischaemia-reperfusion injury
Journal: Acta cirurgica brasileira
Database: PERIÓDICA
System number: 000271971
ISSN: 0102-8650
Authors: 1


2




Institutions: 1Universidade Federal de Mato Grosso, Cuiaba, Mato Grosso. Brasil
2Hospital Santa Rosa, Cuiaba, Mato Grosso. Brasil
Year:
Volumen: 22
Pages: 34-39
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Experimental
English abstract PURPOSE: The aim of this study was to investigate whether the hind limbs or intestinal tract is the most important initiator of the inflammatory response secondary aortic clamping and hind limb ischemia/reperfusion injury. METHODS: Blood samples of Wistar rats obtained from posterior cava vein, portal vein, and heart cavity during either laparotomy (control group, n=8) or laparotomy + 2 h of aortic clamping and bilateral hind limb ischemia (ischemia group, n=8), or 2 h after ischemia and 2 h of reperfusion (ischemia-reperfusion group, n=8) were assayed for interleukin 6 (IL-6) and C-reactive protein (CRP). RESULTS: Serum IL-6 at the heart (223.6±197.9 [10-832] pg/mL) was higher (p<0.001) than at both portal (133.08±108.52 [4-372] pg/mL) and posterior cava veins (127.58±109.15 [8-388] pg/mL). CRP was not significant different among groups. CONCLUSION: The splanchnic region is also a source of inflammatory response secondary to ischemia and reperfusion of the hind limbs
Portuguese abstract OBJETIVO: Investigar qual o principal mediador da resposta inflamatória na lesao de isquemia/reperfusão após clampeamento da aorta abdominal e isquemia dos membros inferiores: o intestine ou as extremidades inferiores. MÉTODOS: amostra de sangue de ratos Wistar coletados da cava posterior, porta e cavidade cardíaca during tanto laparotomia (grupo controle n=8) ou laparotomia + 2 horas de clampeamento aórtico e isquemia bilateral de membros posteriores (grupo isquemia n=8), ou 2 h de isquemia seguido por 2 horas de reperfusão (grupo isquemia/reperfusão n=8), onde foram dosados interleucina 6 e proteína C-reativa. RESULTADOS:Il-6 no coração (223.6±197.9 [10-832] pg/mL) foi maior (p<0.001) tanto na veia porta (133.08±108.52 [4-372] pg/mL) quanto na veia cava posterior (127.58±109.15 [8-388] pg/mL). PCR não foi significativamente diferente entre os grupos. CONCLUSÃO: o trato intestinal foi responsável pela resposta inflamatória secundária a lesão de isquemia/reperfusão
Disciplines: Medicina
Keyword: Cirugía,
Medicina experimental,
Inmunología,
Inflamación,
Isquemia,
Interleucina-6,
Proteína C reactiva,
Mucosas,
Aneurismas,
Aorta abdominal
Keyword: Medicine,
Experimental medicine,
Surgery,
Inflammation,
Ischemia,
Interleukin-6,
C reactive protein,
Mucosa,
Aneurysms,
Abdominal aorta,
Immunology
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