| Revista: | The journal of venomous animals and toxins |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000298614 |
| ISSN: | 0104-7930 |
| Autores: | Guidolin, R1 Stephano, M.A2 Morais, J.F Sakaguti, E.H3 Prado, S.M.A4 Fratelli, F Vancetto, M.D.C Higashi, H.G |
| Instituciones: | 1Instituto Butantan, Divisao de Desenvolvimento Tecnologico e Producao, Sao Paulo. Brasil 2Instituto Butantan, Secao de Processamento de Plasmas Hiperimunes, Sao Paulo. Brasil 3Instituto Butantan, Secao de Obtencao de Plasmas Hiperimunes, Sao Paulo. Brasil 4Instituto Butantan, Secao de Vacunas Anaerobicas, Sao Paulo. Brasil |
| Año: | 1998 |
| Volumen: | 4 |
| Número: | 1 |
| Paginación: | 70-78 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | The present investigation reveals the possibility of simultaneous immunization of horses with Bothrops or Crotalus snake venoms and Tetanus antigens for the production of anti-Bothrops-Tetanus or anti-Crotalus-Tetanus mixed serum, with high titers of the respective specific antibodies. Bothrops antivenoms with an average neutralizing titer of 4.16 mg venom/ml were obtained from plasma of horses with titers lower than 0.5 mg venom/ml when Tetanus antigens were not used. This suggests the existence of a synergism between Bothrops venoms and Tetanus antigens in the stimulation of the antibody response. The pooled plasma of the animals had a neutralizing titer of 21.0 mg/ml reference Bothrops venoms and 3,300 IU/ml to Tetanus antigens after purification by enzymatic digestion and ammonium sulphate precipitation. These experiments lead us to conclude that Bothrops envenomation therapy can be successfully performed using anti-Bothrops-Tetanus serum also serving as Tetanus prophylaxis. Anti-Crotalus-Tetanus serum can also be produced, although it is not of medical interest as Crotalus envenomation rarely results in local necrotizing lesions |
| Disciplinas: | Biología |
| Palabras clave: | Inmunología, Antisueros, Inmunización, Caballos, Veneno de víbora, Tétanos, Bothrops |
| Keyword: | Biology, Immunology, Antisera, Immunization, Horses, Snake venom, Tetanus, Bothrops |
| Texto completo: | Texto completo (Ver HTML) |
