Revue: | The journal of venomous animals and toxins |
Base de datos: | PERIÓDICA |
Número de sistema: | 000298550 |
ISSN: | 0104-7930 |
Autores: | Rocha Campos, A.C.M1 Freitas, M.C.W. de Yamguchi, I.K Marcelino, J.R Dias da Silva, W |
Instituciones: | 1Instituto Butantan, Laboratorio de Imunoquimica, Sao Paulo. Brasil |
Año: | 1996 |
Volumen: | 2 |
Número: | 2 |
Paginación: | 106-120 |
País: | Brasil |
Idioma: | Inglés |
Tipo de documento: | Artículo |
Enfoque: | Experimental, aplicado |
Resumen en inglés | Phospholipase A2 (PLA2), a component of most snake venom toxins, cleaves 3-sn-phosphoglycerides releasing lysophosphatidyl-choline. The indirect quantitative assay method for PLA2 was standardized for specific antivenom titration in a fast and sensitive assay by the similarity with the hemolysis induced by PLA2 and by complement system in sheep erythrocytes. The curves obtained by plotting the degree of hemolysis against the doses of snake venom are concave to the abscissa axis following an equation similar to that previously described for the hemolysis induced by the C system. We observed that venoms of some Bothrops, Crotalus and Micrurus species contained around 1 x 10 to 10 Z/mg of venom, while the venom of Naja contained over one million Z/mg. Antibodies against PLA2 were titrated by incubating amounts of venom predetermined to give 1 to 5 Z with various dilutions of the antivenoms, and the remaining active PLA2 was determined in the hemolytic assay. We observed the following: a) the antivenoms contained specific antibodies against the PLA2 present in the corresponding venoms; b) cross-reactivity was not detected among PLA2 epitopes from venoms and nonspecific antivenoms; and c) the assay quantitatively performed determined the specific antibodies directed to epitopes on the molecule of PLA2. The method described in this paper is highly specific, sensitive and reproducible, besides being fast and inexpensive |
Disciplinas: | Medicina |
Palabras clave: | Toxicología, Inmunología, Veneno de víbora, Antivenenos, Inmunoensayo, Hemólisis indirecta, Fosfolipasa A2, Inhibición enzimática |
Keyword: | Medicine, Toxicology, Immunology, Snake venom, Antivenoms, Immunoassay, Indirect hemolysis, Phospholipase A2, Enzymatic inhibition |
Texte intégral: | Texto completo (Ver HTML) |