| Revista: | The journal of venomous animals and toxins including tropical diseases |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000298977 |
| ISSN: | 1678-9199 |
| Autores: | Vonorax, E.J1 Tyler, M.I2 Atkinson, R.K3 Howden, M.E.H |
| Instituciones: | 1Deakin University, School of Biological and Chemical Sciences, Geelong, Victoria. Australia 2Macquarie University, Australian Proteome Analysis Facility, Sydney, Nueva Gales del Sur. Australia 3University of Southern Queensland, School of Applied Science, Toowoomba, Queensland. Australia |
| Año: | 2006 |
| Periodo: | Abr |
| Volumen: | 12 |
| Número: | 2 |
| Paginación: | 215-233 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental |
| Resumen en inglés | Australian funnel-web spiders are relatively large primitive hunting spiders. Male Atrax robustus spiders have been responsible for a number of human deaths. Venom was collected from the species Hadronyche infensa (Hickman) [female], H. formidabilis [male and female], H. versuta [female], and A. robustus (Cambridge) [male] and was fractionated by high performance liquid chromatography. This resulted in the isolation and purification of a homologous series of 7 insecticidal peptides of relatively low molecular mass (approximately 4kDa). The amino acid sequences of these toxins consisted of 36 or 37 amino acids and were named atracotoxins. For the major bioassay of these toxins, we used the cotton bollworm, Helicoverpa armigera (Hubner), due to the great damage it causes to crops worldwide. These toxins, when injected subcutaneously into fifth or sixth instar larvae of Helicoverpa armigera,were lethal or caused an apparently irreversible writhing. The toxin from H. versuta venom showed no significant toxicity when subcutaneously injected into newborn mice. One of the toxins was found to have a free acid carboxyl terminus. These toxins have great potential as lead compounds for insecticide design or for incorporation in recombinant baculovirus insecticides |
| Disciplinas: | Biología |
| Palabras clave: | Quelicerados, Bioquímica, Arañas, Veneno, Péptidos, Actividad insecticida, Hadronyche, Atrax |
| Keyword: | Biology, Chelicerata, Biochemistry, Spiders, Venom, Peptides, Insecticidal activity, Hadronyche, Atrax |
| Texto completo: | Texto completo (Ver HTML) |
