Detection and characterization of Bacillus cereus isolated from the dialysis fluid



Título del documento: Detection and characterization of Bacillus cereus isolated from the dialysis fluid
Revue: Revista do Instituto de Medicina Tropical de Sao Paulo
Base de datos:
Número de sistema: 000547887
ISSN: 0036-4665
Autores: 1
2
3
1
4
4
1
Instituciones: 1Universidade Federal de Santa Maria, Centro de Ciências da Saúde, Santa Maria, Rio Grande do Sul. Brasil
2Universidade de Cruz Alta, Departamento de Ciências da Saúde, Cruz Alta, Rio Grande do Sul. Brasil
3Universidade Federal de Santa Maria, Centro de Ciências Rurais, Santa Maria, Rio Grande do Sul. Brasil
4Universidade Federal de Santa Maria, Centro de Ciências Naturais e Exatas, Santa Maria, Rio Grande do Sul. Brasil
Año:
Volumen: 64
País: Brasil
Idioma: Inglés
Resumen en inglés In this study, B. cereus was detected in dialysis fluids within international parameters (ultrapure – maximum limit of 0.1 CFU/mL for heterotrophic bacteria count) by analyzing the pellet obtained through the centrifugation method. We also investigated the ability of the B. cereus isolate to form a biofilm at different temperatures, the production of virulence factors, and the susceptibility to commercial antimicrobial agents. This study demonstrated a high ability of B. cereus to persist in the hemodialysis system, which can be explained by its broad ability to produce a biofilm at 25 °C, its relevant production of virulence factors, such as β-hemolysin, lecithinase and cereulide, and its important resistance pattern to antimicrobial drugs. In conclusion, these new findings expand the understanding that this microorganism should not be neglected and new methods for tracking it should be considered.
Keyword: Renal dialysis,
Centrifuge,
Bacillus cereus group,
Biofilm
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