| Revue: | Revista do Instituto de Medicina Tropical de Sao Paulo |
| Base de datos: | |
| Número de sistema: | 000547834 |
| ISSN: | 0036-4665 |
| Autores: | Luchs, Adriana1 Azevedo, Lais Sampaio de1 Souza, Ellen Viana de1 Medeiros, Roberta Salzone1 Souza, Yasmin França Viana Pires de1 Teixeira, Dalane Loudal Florentino2 Carneiro, Thiago Franco de Oliveira2 Alencar, Gabriela Maria Fernandes de3 Morais, Fernanda Lúcia de Sousa Leite3 Pinto, Diana de Fátima Alves4 Okay, Thelma Suely5 Yamamoto, Lidia6 Morais, Vanessa dos Santos6 Araújo, Emerson Luiz Lima7 Leal, Elcio8 Costa, Antonio Charlys da6 |
| Instituciones: | 1Instituto Adolfo Lutz, Centro de Virologia, São Paulo, São Paulo. Brasil 2Estado da Saúde da Paraíba, Laboratório Central de Saúde Pública, João Pessoa, Paraíba. Brasil 3Estado da Saúde da Paraíba, Hospital Infantil Arlinda Marques, João Pessoa, Paraíba. Brasil 4Estado da Saúde da Paraíba, Centro de Informações Estratégicas em Vigilância em Saúde, João Pessoa, Paraíba. Brasil 5Universidade de São Paulo, Faculdade de Medicina, São Paulo, São Paulo. Brasil 6Universidade de São Paulo, Instituto de Medicina Tropical de São Paulo, São Paulo, São Paulo. Brasil 7Ministério da Saúde, Secretaria de Vigilância em Saúde, Distrito Federal, Distrito Federal. Brasil 8Universidade Federal do Pará, Instituto de Ciências Biológicas, Belém, Pará. Brasil |
| Año: | 2022 |
| Volumen: | 64 |
| País: | Brasil |
| Idioma: | Inglés |
| Resumen en inglés | Hand-foot-and-mouth disease (HFMD) is a highly contagious viral disease commonly associated to Enteroviruses (EV). During 2018, Brazil faced massive HFMD outbreaks spread across the country. This study aimed to characterize the EV responsible for the HFMD outbreak that occurred in Paraiba State, Brazilian Northeastern region, in 2018, followed by a phylogenetic analysis to detail information on its genetic diversity. A total of 49 serum samples (one from each patient) collected from children ≤ 15 years old, clinically diagnosed with HFMD were tested for EV using conventional RT-PCR and RT-qPCR. EV infection was confirmed in 71.4% (35/49) of samples. The mean and median ages were 1.83 years and one year old, respectively. Twenty-two EV-positive samples were successfully sequenced and classified as EV-A species; 13 samples were also identified with the CV-A6 genotype. The phylogenetic analysis (VP1 region) of three samples revealed that the detected CV-A6 strains belonged to sub-lineage D3. The CV-A6 strains detected here clustered with strains from South America, Europe and West Asia strains that were also involved in HFMD cases during the 2017-2018 seasons, in addition to the previously detected Brazilian CV-A6 strains from 2012 to 2017, suggesting a global co-circulation of a set of different CV-A6 strains introduced in the country at different times. The growing circulation of the emerging CV-A6 associated with HFMD, together with the detection of more severe cases worldwide, suggests the need for a more intense surveillance system of HFMD in Brazil. In addition, this investigation was performed exclusively on serum samples, and the analysis of whole blood samples should be considered and could have shown advantages when employed in the diagnosis of enteroviral HFMD outbreaks. |
| Keyword: | Hand foot and mouth disease, Enterovirus, Coxsackievirus, Surveillance |
| Texte intégral: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
