Revue: | Revista do Instituto de Medicina Tropical de Sao Paulo |
Base de datos: | |
Número de sistema: | 000537162 |
ISSN: | 0036-4665 |
Autores: | Bes, Taniela Marli1 Nagano, Debora Satie1 Marchi, Ana Paula1 Camilo, Gaspar1 Perdigão Neto, Lauro Vieira1 Martins, Roberta Ruedas1 Levin, Anna Sara1 Costa, Silvia Figueiredo1 |
Instituciones: | 1Universidade de São Paulo, Faculdade de Medicina, São Paulo, São Paulo. Brasil |
Año: | 2021 |
Volumen: | 63 |
País: | Brasil |
Idioma: | Inglés |
Resumen en inglés | The methicillin resistant Staphylococcus aureus (MRSA) is recognized by its ability to acquire and transferring resistance genes through interspecies conjugative plasmids. However, transference of plasmids from Gram-positive cocci to Gram-negative bacilli is not well characterized. In this report, we describe the transfer of a conjugative plasmid carrying qacA from MRSA to Escherichia coli C600. We performed a conjugation experiment using a chlorhexidine resistant MRSA isolate (ST-105/SCCmec type III) carrying the gene qacA and qacC as the donor and a chlorhexidine susceptible E. coli C600 isolate as the receptor. Transconjugants were selected using MacConkey agar plates containing chlorhexidine in concentrations ranging from 0.25 to 16 g.L-1. To genotypically confirm the transfer of the resistance gene, the transconjugants were screened by Polymerase Chain Reaction (PCR) and submitted to Sanger’s sequencing. MRSA isolates successfully transferred the chlorhexidine resistance gene (qacA) to the recipient E. coli strain C600. The E. coli transconjugant exhibited an important reduction of chlorhexidine susceptibility, with MICs increasing from ≤ 0.25 to ≥ 16 g.L-1 after conjugation. The qacA gene was detected by PCR as well as in the Sanger’s sequencing analysis of DNA from transconjugant plasmids. To the best of our knowledge, this is the first report of the plasmid p_8N_qac(MN687830.1) carrying qacA and its transfer by conjugation from a MRSA to an E. coli. These findings increase concerns on the emergence of resistance dissemination across the genus and emphasizes the importance of continuous antiseptic stewardship. |
Keyword: | Chlorhexidine resistance, Conjugation, Conjugative plasmids, QacA |
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