| Revue: | Revista do Instituto de Medicina Tropical de Sao Paulo |
| Base de datos: | |
| Número de sistema: | 000547864 |
| ISSN: | 0036-4665 |
| Autores: | Benigno, Tiago Gomes da Silva1 Ribeiro Junior, Howard Lopes2 Azevedo, Orleâncio Gomes Ripardo de1 Pinheiro, Ronald Feitosa1 Oliveira, Roberta Taiane Germano de1 Maciel, Felipe Silva1 Oliveira, Edson Luiz de1 Queiroz, Dulciene Maria Magalhães3 Braga, Lucia Libanez Bessa Campelo1 |
| Instituciones: | 1Universidade Federal do Ceará, Faculdade de Medicina, Fortaleza, Ceará. Brasil 2Hospital de Câncer de Barretos, Centro de Pesquisa em Oncologia Molecular, Barretos, São Paulo. Brasil 3Universidade Federal de Minas Gerais, Faculdade de Medicina, Belo Horizonte, Minas Gerais. Brasil 4Universidade Federal do Ceará, Instituto Nacional de Ciência e Tecnologia de Biomedicina do Semiárido Brasileiro, Fortaleza, Ceará. Brasil |
| Año: | 2022 |
| Volumen: | 64 |
| País: | Brasil |
| Idioma: | Inglés |
| Resumen en inglés | The increase of H. pylori resistance to clarithromycin is a concern. This study evaluated the prevalence of H. pylori’s primary resistance to clarithromycin and its association with virulence factors in adult dyspeptic patients and asymptomatic children. The gastric mucosa from patients (153 gastritis, 24 gastric cancer, 21 peptic ulcer) and gastric juice obtained by string test from 24 H. pylori and 23S rRNA positive asymptomatic children were included. The clarithromycin resistance was assessed by TaqMan RT-PCR 23S rRNA point mutations, A2142G and/or A2143G, and H. pylori virulence markers by PCR. Overall, the clarithromycin resistance was 14.4% (32/222), 14.2% in adults, and 12% in children, whereas origin, gender, and disease were not distinctive factors. The most prevalent point mutation was A2143G (62.5%). The point mutation was significantly less frequent in cagA-positive (11.4%) than in cagA-negative (23.6%) strains (p=0.03 OR = 0.4 95%CI = 0.19 - 0.91) as well as in cagE-positive (10.2%), cagE-negative (21.2%) (p=0.03 OR: 0.4 I.C:0.20-0.91). No difference was found in iceA or vacA alleles genotypes. Primary resistance to clarithromycin was lower than that reported in Southeast Brazil. The cagA and cagE positive H. pylori samples have few point mutations suggesting that individuals infected with virulent strains may be more susceptible to anti-H. pylori treatment. |
| Keyword: | H, Pylori, Virulence factors, Clarithromycin-resistance |
| Texte intégral: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
