| Revue: | Revista de investigación clínica |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000453199 |
| ISSN: | 0034-8376 |
| Autores: | Valdés Peregrina, Estefanie N1 Sánchez Hernández, Beatriz E1 Gamboa Domínguez, Armando1 |
| Instituciones: | 1Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Departamento de Patología, Ciudad de México. México |
| Año: | 2020 |
| Periodo: | Sep-Oct |
| Volumen: | 72 |
| Número: | 5 |
| Paginación: | 316-322 |
| País: | México |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Analítico, descriptivo |
| Resumen en inglés | Hepatocarcinogenesis has a variety of risk factors. In Mexico City, autopsies found 14% of hepatocellular carcinomas (HCCs) without cirrhosis. Objective: The objective of the study was to explore if HCCs carry the TP53 R249S mutation that has linked them to aflatoxin exposure and describe the associated risk factors. Methods: A retrospective review of consecutive cases of HCC was performed. Exposure to hepatotropic viruses, alcoholism, metabolic diseases, diabetes mellitus, and hypertension, as well as episodes of ascites, portal hypertension, and body mass index were retrieved. Slides were re-reviewed, macrodissected and DNA was extracted. TP53 exon 7 was amplified, purified, and used as a template for sequencing. Results: In 14 years, 74 HCCs were identified in 1863 (4%) consecutive liver biopsies. No data were available in five excluded patients; the rest was submitted to exon 7 screening. Patients had a median age of 62 years, and 46 (67%) were male. Stage 4 fibrosis was observed in 46 patients (67%) and their associated risk factors were hepatitis C virus (39%, 18/46), alcoholism (20%, 9/46), hepatitis B virus (2%, 1/46), and 18 were cryptogenic. Fibrosis stage 3 or lower was observed in 23 (33%) patients without demonstrated liver disease; 8/23 had diabetes and 6/23, systemic hypertension. Steatohepatitic variants of HCC were observed in 4 and in 5, the remnant liver had steatohepatitis. A 238-bp fragment was obtained in each tumor without the expected TP53 R249S mutation. Conclusions: There was no evidence of aflatoxin exposure in HCCs, with and without the known “classical” risk factors. One-third of non-cirrhotic HCCs had steatohepatitis or conditions associated to metabolic syndrome |
| Disciplinas: | Medicina |
| Palabras clave: | Oncología, Gastroenterología, Factores de riesgo, Carcinoma hepatocelular, Síndrome metabólico, Aflatoxinas, Esteatohepatitis, Diabetes mellitus, Dislipidemia |
| Keyword: | Oncology, Gastroenterology, Risk factors, Hepatocellular carcinoma, Metabolic syndrome, Aflatoxins, Steatohepatitis, Diabetes mellitus, Dyslipidemia |
| Texte intégral: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
