Complete Screening of Exons 2, 3, and 4 of KRAS and NRAS Genes Reveals a Higher Number of Clinically Relevant Mutations than Food and Drug Administration Quantitative Polymerase Chain Reaction-Based Commercial Kits
Título del documento: Complete Screening of Exons 2, 3, and 4 of KRAS and NRAS Genes Reveals a Higher Number of Clinically Relevant Mutations than Food and Drug Administration Quantitative Polymerase Chain Reaction-Based Commercial Kits
Revue: Revista de investigación clínica
Base de datos: PERIÓDICA
Número de sistema: 000453195
ISSN: 0034-8376
Autores: 1
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Instituciones: 1Vitagénesis S.A. de C.V., Laboratorio de Genética, Monterrey, Nuevo León. México
Año:
Periodo: Nov-Dic
Volumen: 72
Número: 6
Paginación: 337-343
País: México
Idioma: Inglés
Tipo de documento: Nota breve o noticia
Enfoque: Aplicado, descriptivo
Resumen en inglés The presence of clinically relevant mutations in KRAS and NRAS genes determines the response of anti-epidermal growth factor receptor antibody therapy for metastatic colorectal cancer (mCRC). The only quantitative polymerase chain reaction (qPCR)-based diagnostic tests approved by the Food and Drug Administration (FDA) screen merely for mutations in codons 12 and 13 of KRAS. Objective: The objective of the study was to study the frequency of clinically relevant mutations in KRAS and NRAS genes that are not included in FDA-approved qPCR tests. Methods: Formalin-fixed paraffin-embedded tumor specimens from 1113 mCRC Mexican patients from different health institutions across the country were analyzed by Sanger sequencing for KRAS mutations in exons 2, 3, and 4. Furthermore, 83 were analyzed in exons 2, 3, and 4 of NRAS. Results: From the specimens tested for KRAS, 33.69% harbored a mutation. From these, 71.77% were in codon 12 and 27.69% in codon 13 (both located in exon 2). Codons 59 (exon 3) and 146 (exon 4) accounted for the remaining 0.54%. From the 83 specimens, in which NRAS was analyzed, three mutations were found in codon 12 (3.61%). Approximately 6% of RAS mutated specimens would have been falsely reported as RAS wild type if an FDA-approved qPCR diagnostic test had been used. Conclusions: While these kits based on qPCR can be very practical and highly sensitive, their mutation coverage ignores mutations from poorly genetically characterized populations
Disciplinas: Medicina
Palabras clave: Oncología,
Diagnóstico,
Cáncer colorrectal,
Diagnóstico molecular,
Reacción en cadena de la polimerasa (PCR),
Secuenciación de Sanger
Keyword: Oncology,
Diagnosis,
Colorectal cancer,
Molecular diagnosis,
Polymerase chain reaction (PCR),
Sanger sequencing
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