| Revue: | Revista de investigación clínica |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000453203 |
| ISSN: | 0034-8376 |
| Autores: | Cabrera Mendoza, Brenda1 Martínez Magaña, José J1 Genis Mendoza, Alma D1 Monroy Jaramillo, Nancy2 Walss Bass, Consuelo3 Fries, Gabriel R3 García Dolores, Fernando4 López Armenta, Mauro4 Flores, Gonzalo5 Vázquez Roque, Rubén A5 Nicolini, Humberto1 |
| Instituciones: | 1Instituto Nacional de Medicina Genómica, Laboratorio de Genómica de Enfermedades Psiquiátricas y Neurodegenerativas, Ciudad de México. México 2Instituto Nacional de Neurología y Neurocirugía Manuel Velasco Suárez, Departamento de Genética, Ciudad de México. México 3University of Texas, Health Science Center at Houston, Houston, Texas. Estados Unidos de América 4Instituto de Ciencias Forenses, Ciudad de México. México 5Benemérita Universidad Autónoma de Puebla, Instituto de Fisiología, Puebla. México |
| Año: | 2020 |
| Periodo: | Sep-Oct |
| Volumen: | 72 |
| Número: | 5 |
| Paginación: | 283-292 |
| País: | México |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | Background: Gene expression alterations have been implicated in suicide pathology. However, the study of the regulatory effect of DNA methylation on gene expression in the suicidal brain has been restricted to candidate genes. Objective: The objective of the study was to identify genes whose expression levels are correlated with DNA methylation in the prefrontal cortex of suicides. Methods: Postmortem prefrontal cortex samples from 21 suicides and six non-suicides were collected. Transcriptomic and DNA methylation profiles were evaluated with microarrays; cis correlations between gene expression and CpG methylation were screened. We then analyzed the presence of transcription factor (TF) binding sites (TFBS) at CpG sites correlated with gene expression. Gene expression of TFs involved in neurodevelopmental binding to predicted TFBS was determined in the BrainSpan database. Results: We identified 22 CpG sites whose methylation levels correlated with gene expression in the prefrontal cortex of suicides. Genes annotated to identified CpG sites were involved in neurodevelopment (BBS4, NKX6-2, AXL, CTNND1, and MBP) and polyamine metabolism (polyamine oxidase [PAOX]). Such correlations were not detected in the non-suicide group. Nine TFs (USF1, TBP, SF1, NRF1, RFX1, SP3, PKNOX1, MAZ, and POU3F2) showed differential expression in pre- and post-natal developmental periods, according to BrainSpan database. Conclusions: The integration of different omic technologies provided novel candidates for the investigation of genes whose expression is altered in the suicidal brain and their potential regulatory mechanisms |
| Disciplinas: | Medicina |
| Palabras clave: | Neurología, Psiquiatría, Genética, Suicidio, Metilación del ADN, Expresión génica, Corteza cerebral, Microarreglos genéticos, Factores de transcripción, Neurodesarrollo |
| Keyword: | Neurology, Psychiatry, Genetics, Suicide, DNA methylation, Gene expression, Cerebral cortex, Genetic microarrays, Transcription factors, Neurodevelopment |
| Texte intégral: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
