Identification of candidate genes associated with milk yield trait in buffaloes ( Bubalus bubalis ) by restriction-site-associated DNA sequencing
Título del documento: Identification of candidate genes associated with milk yield trait in buffaloes ( Bubalus bubalis ) by restriction-site-associated DNA sequencing
Revista: Revista Brasileira de Zootecnia
Base de datos: PERIÓDICA
Número de sistema: 000445515
ISSN: 1516-3598
Autores: 1
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Instituciones: 1Yangzhou University, College of Bioscience and Biotechnology, Yangzhou, Jiangsu. China
2Yangzhou University, College of Animal Science and Technology, Yangzhou, Jiangsu. China
3National Research Center, Genetic Engineering and Biotechnology Division, Giza. Egipto
Año:
Volumen: 49
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, analítico
Resumen en inglés The objectives of our present study included the screening of single nucleotide polymorphisms (SNP) that show significant differences in allelic frequencies between two buffalo populations (Egyptian and Chinese buffaloes), categorization of functional genes associated with these SNP by gene ontology, and pathway analyses to further understand their potential values as candidate genes closely associated with milk yield trait in buffaloes. In this study, double digest restriction-site associated DNA sequencing was performed on Illumina HiSeq 2500 platform for 20 and 25 female buffaloes from Egypt and China, respectively. Approximately 118 Gb of sequencing data were obtained, and a total of 110,129 and 150,535 putative SNP were detected in Egyptian and Chinese buffaloes, respectively. Focused only on those SNP that differed significantly in allelic frequencies between the two populations, we found that genes associated with these SNP were significantly over-represented in the ionotropic glutamate receptor pathway, the endothelin signaling pathway, and the gonadotropin-releasing hormone receptor pathway, which contained a total of 29 genes. Of these, nine genes ( ADCY5, CACNA1A, CREB1, INHBA , INHBB, PIK3R1, PLCB1, PRKCE , and SMAD2 ) participating in the hormonal regulation of lactation, were considered to be promising candidate genes worthy of further investigations for favorable alleles associated with milk yield. Our results provide useful information about genetic variations in Egyptian and Chinese buffaloes. The potential influences of nine candidate genes and their associated SNP on milk yield need to be validated in more buffalo populations
Disciplinas: Medicina veterinaria y zootecnia
Palabras clave: Bovinos,
Reproducción y genética animal,
Búfalas,
Bubalus bubalis,
Producción de leche,
ADN
Keyword: Bovines,
Animal reproduction and genetics,
Buffaloes,
Bubalus bubalis,
Milk production,
DNA
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