| Revue: | Revista Brasileira de Zootecnia |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000444063 |
| ISSN: | 1516-3598 |
| Autores: | Blecha, Isabella Maiumi Zaidan1 Sousa, Isadora Inácio1 Ferreira, Anna Beatriz Robottom2 Feijó, Gelson Luis Dias3 Torres-Junior, Roberto Augusto de Almeida3 Siqueira, Fabiane3 |
| Instituciones: | 1Universidade Federal de Mato Grosso do Sul, Faculdade de Medicina Veterinaria e Zootecnia, Campo Grande, Mato Grosso do Sul. Brasil 2Empresa Brasileira de Pesquisa Agropecuaria, Agroindustria de Alimentos, Rio de Janeiro. Brasil 3Empresa Brasileira de Pesquisa Agropecuaria, Gado de Corte, Campo Grande, Mato Grosso do Sul. Brasil |
| Año: | 2019 |
| Volumen: | 48 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, analítico |
| Resumen en inglés | The objectives of this study were to genotype single nucleotide polymorphisms (SNP) AF159246:g.2959A>G (CAST/DdeI) and AF248054.2:g.6545C>T (CAPN4751) in beef cattle by PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment Length Polymorphism), using the restriction enzyme DdeI for both SNP, and describe the use of these genotyping methodologies for the first time. For the SNP located in the CAST gene, new primers were designed, and for the SNP of the CAPN1 gene, the same primers previously described in the literature were used. Bonsmara, Caracu, Senepol, Nelore, and Angus bulls were chosen from among the most used bulls in breeding programs according to their genealogy and the lowest possible degree of parentage between them to ensure an experimental sample representative of the genetic variability in each breed. For the CAST and CAPN1 genes, respectively, the following number of animals were analyzed: Bonsmara (n = 25/22), Caracu (n = 25/26), Senepol (n = 25/24), Nelore (n = 26/26), and Angus (n = 25/24). The accuracy of these methodologies was confirmed by direct sequencing of PCR products generated for the two polymorphisms. The new primers developed for CAST/DdeI SNP detection and the use of DdeI enzyme for CAPN4751 SNP detection were effective in genotyping, since no inconclusive genotypes were observed for these genes. Thus, the genotyping of beef cattle using the PCR-RFLP technique for CAST and CAPN1 genes is robust, relatively inexpensive, and easy to perform in any basic molecular biology laboratory. If the association of these markers with traits of economic interest in beef cattle is confirmed in new studies, these methodologies may contribute to the selection of animals with superior genetics, i.e., with the potential to produce better-quality meat, either by marker-assisted selection or by the inclusion of these polymorphisms in high-density marker panels |
| Disciplinas: | Medicina veterinaria y zootecnia |
| Palabras clave: | Bovinos, Reproducción y genética animal, Genes CAST, Genes CAPN1, Marcaje genético, Polimorfismo, Ganado de engorda |
| Keyword: | Bovines, Animal reproduction and genetics, CAST genes, CAPN1 genes, Genetic labelling, Polymorphism, Beef cattle |
| Texte intégral: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
