| Revue: | Quimica nova |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000313159 |
| ISSN: | 0100-4042 |
| Autores: | Oliveira, Ines Rosane Welter Zwirtes de1 Vieira, Iolanda Cruz |
| Instituciones: | 1Universidade Federal de Santa Catarina, Departamento de Quimica, Florianopolis, Santa Catarina. Brasil |
| Año: | 2006 |
| Periodo: | Sep |
| Volumen: | 29 |
| Número: | 5 |
| Paginación: | 932-939 |
| País: | Brasil |
| Idioma: | Portugués |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | Biosensors were developed by immobilization of gilo (Solanum gilo) enzymatic extract on chitosan biopolymers using three different procedures: glutaraldehyde, carbodiimide/glutaraldehyde and epichlorohydrin/glutaraldehyde. The best biosensor performance was obtained after the immobilization of peroxidase on chitosan with epichlorohydrin/glutaraldehyde. Linear analytical curves for hydroquinone concentrations from 2.5x10-4 to 4.5x10-3 mol L-1 with a detection limit of 2.0x10-6 mol L-1 and recovery of hydroquinone ranging from 95.1 to 105% were obtained. The relative standard deviation was < 1.0 % for a solution of 3.0x10-4 mol L-1 hydroquinone and 2.0x10-3 mol L-1 hydrogen peroxide in 0.1 mol L-1 phosphate buffer solution at pH 7.0 (n=8). The lifetime of this biosensor was 6 months (at least 300 determinations) |
| Disciplinas: | Química |
| Palabras clave: | Bioquímica, Biotecnología, Inmovilización enzimática, Biosensores, Peroxidasa, Quitosano |
| Keyword: | Chemistry, Biochemistry, Biotechnology, Enzyme immobilization, Peroxidase, Biosensors, Chitosan |
| Texte intégral: | Texto completo (Ver HTML) |
