Ultrastructural observation of the airways of recovered and susceptible pigs after inoculation with Mycoplasma hyopneumoniae



Título del documento: Ultrastructural observation of the airways of recovered and susceptible pigs after inoculation with Mycoplasma hyopneumoniae
Revista: Pesquisa veterinaria brasileira
Base de datos: PERIÓDICA
Número de sistema: 000301397
ISSN: 0100-736X
Autores: 1
2
3
4
Instituciones: 1Universidade Federal de Santa Maria, Departamento de Patologia Veterinaria, Santa Maria, Rio Grande do Sul. Brasil
2Purdue University, Department of Pathobiology, West Lafayette, Indiana. Estados Unidos de América
3Purdue University, Department of Veterinary Anatomy, West Lafayette, Indiana. Estados Unidos de América
4Purdue University, Department of Veterinary Clinical Sciences, West Lafayette, Indiana. Estados Unidos de América
Año:
Periodo: Ene-Mar
Volumen: 18
Número: 1
Paginación: 1-7
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, analítico
Resumen en inglés To determine the morphological differences in the epithelium of the airways of recovered and susceptible pigs after Mycoplasma hyopneumoniae challenge, twenty-four 4-week-old M. hyopneumoniae-free pigs were intratracheally inoculated with 107ccu/ml of a pure low-passaged culture of the P5722-3 strain of M. hyopneumoniae challenge material. Eight pigs (group I) were challenged at the beginning of the experiment and rechallenged 3 months later. Group II pigs were also challenged at the beginning of the experiment and necropsied 3 months later. Group III pigs were challenged at the same time as the rechallenge of group I pigs. Eight nonchallenged pigs served as controls (group IV). Three days after the second challenge of group I and the first challenge of group III, and every 3 and 4 days thereafter, two pigs from each group were euthanatized by electrocution and necropsied. Samples of bronchi and lung tissue were examined using light and electron microscopy (SEM and TEM). Macroscopic lesions were observed in the lungs of all group III pigs (average = 4.74%) and were characterized by purple-red areas of discoloration and increased firmness affecting the cranioventral aspect of the lungs. Macroscopic lesions of pneumonia in groups I and II were minimal (less than 1%). There were no gross lesions of pneumonia in control (group IV) pigs. Microscopic lesions were characterized by hyperplasia of the peribronchial lymphoid tissue and mild neutrophilic infiltrates in alveoli. Electron microscopy showed patchy areas with loss of cilia and pr
Disciplinas: Medicina veterinaria y zootecnia,
Biología
Palabras clave: Medicina veterinaria,
Porcinos,
Biología celular,
Parasitología,
Pneumonia enzoótica,
Mycoplasma hyopneumoniae,
Vías aéreas
Keyword: Veterinary medicine and animal husbandry,
Biology,
Swine,
Veterinary medicine,
Cell biology,
Parasitology,
Mycoplasma hyopneumoniae,
Enzootic pneumonia,
Airways
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