| Revista: | Pesquisa veterinaria brasileira |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000301483 |
| ISSN: | 0100-736X |
| Autores: | Madruga, Claudio R1 Leal, Cassia R.B2 Ferreira, Alda M.T Araujo, Flabio R Bonato, Ana L.V Kessler, Raul H Schenk, Maria A.M Soares, Cleber O |
| Instituciones: | 1Empresa Brasileira de Pesquisa Agropecuaria, Gado de Corte, Campo Grande, Mato Grosso do Sul. Brasil 2Universidade Catolica Dom Bosco, Campo Grande, Mato Grosso do Sul. Brasil |
| Año: | 2002 |
| Periodo: | Oct-Dic |
| Volumen: | 22 |
| Número: | 4 |
| Paginación: | 153-160 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, analítico |
| Resumen en inglés | A molecular epidemiological study was performed with Babesia bigemina isolates from five geographical regions of Brazil. The genetic analysis was done with random amplification of polymorphic DNA (RAPD), repetitive extragenic palindromic elements-polymerase chain reaction (REP-PCR) and enterobacterial repetitive intergenic consensus sequences-polymerase chain reaction (ERIC-PCR) that showed genetic polymorphism between these isolates and generated fingerprinting. In RAPD, ILO872 and ILO876 primers were able to detect at least one fingerprinting for each B. bigemina isolate. The amplification of B. bigemina DNA fragments by REP-PCR and ERIC-PCR gave evidence for the presence in this haemoprotozoan of the sequences described previously in microorganisms of the bacterial kingdom. For the first time it was demonstrated that both techniques can be used for genetic analysis of a protozoan parasite, although the ERIC-PCR was more discriminatory than REP-PCR. The dendogram with similarity coefficient among isolates showed two clusters and one subcluster. The Northeastern and Mid-Western isolates showed the greatest genetic diversity, while the Southeastern and Southern isolates were the closest. The antigenic analysis was done through indirect fluorescent antibody technique and Western blotting using a panel of monoclonal antibodies directed against epitopes on the merozoite membrane surface, rhoptries and membrane of infected erythrocytes. As expected, the merozoite variable surface antigens, major surface antigen (MSA)-1 and MSA-2 showed |
| Disciplinas: | Medicina veterinaria y zootecnia, Biología |
| Palabras clave: | Bovinos, Parasitología, Biología molecular, Epidemiología, Babesia bigemina, Fisiografía, Brasil, Polimorfismo genético, Antigenicidad |
| Keyword: | Veterinary medicine and animal husbandry, Biology, Bovines, Parasitology, Molecular biology, Epidemiology, Babesia bigemina, Physiography, Brazil, Genetic polymorphism, Antigenicity |
| Texto completo: | Texto completo (Ver HTML) |
