| Revue: | Memorias do Instituto Oswaldo Cruz |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000446501 |
| ISSN: | 0074-0276 |
| Autores: | Fujimori, Mahyumi1 Almeida, Arleana do Bom Parto Ferreira de2 Barrouin Melo, Stella Maria3 Cortez, Luiz Ricardo Paes de Barros4 Duthie, Malcolm Scott5 Hiramoto, Roberto Mitsuyoshi6 Pinho, Flaviane Alves de3 Reed, Steven Gregory5 Sousa, Valéria Régia Franco2 Souza, Nazaré Fonseca7 Soares, Rodrigo Martins8 Tolezano, José Eduardo6 Sanchez, Maria Carmen Arroyo1 Goto, Hiro1 |
| Instituciones: | 1Universidade de Sao Paulo, Faculdade de Medicina, Sao Paulo. Brasil 2Universidade Federal de Mato Grosso, Faculdade de Medicina Veterinaria, Cuiaba, Mato Grosso. Brasil 3Universidade Federal da Bahia, Hospital-Escola de Medicina Veterinaria, Salvador, Bahia. Brasil 4Secretaria Municipal de Saude de Bauru, Departamento de Saude Coletiva, Bauru, Sao Paulo. Brasil 5Host Directed Therapeutics, Seattle, Washington. Estados Unidos de América 6Instituto Adolfo Lutz, Centro de Parasitologia e Micologia, Sao Paulo. Brasil 7Universidade Federal Rural da Amazonia, Instituto da Saude e Producao Animal, Belem, Para. Brasil 8Universidade de Sao Paulo, Faculdade de Medicina Veterinaria e Zootecnia, Sao Paulo. Brasil |
| Año: | 2021 |
| Volumen: | 116 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Aplicado, descriptivo |
| Resumen en inglés | BACKGROUND Dogs are the main peridomiciliary reservoir of Leishmania infantum thus the correct diagnosis of infection is essential for the control of the transmission and treatment as well. However, the diagnosis is based on serological assays that are not fully effective. OBJECTIVE We aimed to establish an effective serological assay for the diagnosis of L. infantum infected dogs using Leishmania-derived recombinant antigens. METHODS Leishmania derived rK39-, rK28-, rKR95-based enzyme-linked immunosorbent assay (ELISA) was standardized using symptomatic and asymptomatic L. infantum-infected dogs. Then 2,530 samples from inquiry in endemic areas for VL were evaluated and the results compared with recommended assays by the Brazilian Ministry of Health (MH algorithm). Further samples from a cohort of 30 dogs were searched. FINDINGS For rK39-, rK28- and rKR95-ELISA the sensitivity was around 97% and specificity 100%. The positivity of these three ELISA in the inquiry samples was 27-28%, around 10% higher than the assays currently in use. When cohort samples were searched, we observed likely false-negative results (> 65%) with supposedly negative samples that turned positive six months later with the assays in use (MH algorithm). MAIN CONCLUSIONS For the diagnosis of L. infantum-infected dogs, rK39-based ELISA showed better diagnostic performance than other assays in use in Brazil and worldwide |
| Disciplinas: | Medicina |
| Palabras clave: | Parasitología, Diagnóstico, Leishmaniasis visceral canina, Antígenos recombinantes, ELISA |
| Keyword: | Parasitology, Diagnosis, Canine visceral leishmaniasis, Recombinant antigens, ELISA |
| Texte intégral: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
