| Revue: | Memorias do Instituto Oswaldo Cruz |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000452400 |
| ISSN: | 0074-0276 |
| Autores: | Bolais, Paula F1 Galal, Lokman1 Cronemberger, Cecília2 Pereira, Fabiane de Aguiar2 Barbosa, Alynne da Silva3 Dib, Laís Verdan3 Amendoeira, Maria Regina Reis4 Dardé, Marie-Laure1 Mercier, Aurélien1 |
| Instituciones: | 1Universite de Limoges, Institute of Epidemiology and Tropical Neurology, Limoges, Haute-Vienne. Francia 2Instituto Chico Mendes de Conservacao da Biodiversidade, Parque Nacional da Serra dos Orgaos, Teresopolis, Rio de Janeiro. Brasil 3Universidade Federal Fluminense, Instituto Biomedico, Niteroi, Rio de Janeiro. Brasil 4Fundacao Oswaldo Cruz, Instituto Oswaldo Cruz, Rio de Janeiro. Brasil 5Centre Hospitalier-Universitaire Dupuytren, Centre National de Reference, Limoges, Haute-Vienne. Francia |
| Año: | 2022 |
| Volumen: | 117 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Analítico, descriptivo |
| Resumen en inglés | Toxoplasma gondii is a apicomplexan parasite of virtually all warm-blooded species. All true cats (Felidae) can act as definitive hosts for this parasite by shedding resistant oocysts into the environment. However, the patterns of oocysts shedding are only partially understood in domestic cats and largely unknown in wild felids. OBJECTIVES We carried out molecular analysis of 82 faecal samples from wild felids collected in the Serra dos Órgãos National Park (Parnaso), Rio de Janeiro, Brazil. METHODS We screened samples for T. gondii DNA using a quantitative polymerase chain reaction (qPCR) targeting the 529bp DNA fragment. Polymerase chain reaction (PCR)-positive samples were genotyped using 15 microsatellite markers. RESULTS Only one faecal sample from a Puma yagouaroundi was PCR-positive [cycle threshold (Ct) = 26.88]. This sample was contaminated by a T. gondii strain of BrIII lineage, a common lineage in domestic animals from Brazil. MAIN CONCLUSIONS This first report of T. gondii in faeces of wild South American felids in their natural environment indicates infrequent oocyst shedding and suggests a role of acquired immunity in limiting re-excretion as in domestic cats. The presence of a domestic strain of T. gondii in a faecal sample from a wild felid at very low concentrations (not detected by microscopy) is consistent with the hypothesis of host-parasite co-adaptations limiting the circulation of T. gondii strains between domestic and wild environments |
| Disciplinas: | Biología |
| Palabras clave: | Parasitología, Mamíferos, Felinos, Parásitos, Diversidad genética, Oocistos, Toxoplasma gondii, Felidae, Brasil |
| Keyword: | Parasitology, Mammals, Felines, Parasites, Genetic diversity, Oocysts, Toxoplasma gondii, Felidae, Brazil |
| Texte intégral: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
