| Revue: | Memorias do Instituto Oswaldo Cruz |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000446392 |
| ISSN: | 0074-0276 |
| Autores: | Pita, Sebastián1 Gómez Palacio, Andrés2 Lorite, Pedro3 Dujardin, Jean Pierre4 Chávez, Tamara5 Villacís, Anita G6 Galvão, Cleber7 Panzera, Yanina1 Calleros, Lucía1 Pereyra Mello, Santiago1 Burgueño Rodríguez, Gabriela8 Panzera, Francisco1 |
| Instituciones: | 1Universidad de la República, Facultad de Ciencias, Montevideo. Uruguay 2Universidad Pedagógica y Tecnológica de Colombia, Laboratorio de Investigación en Genética Evolutiva, Tunja, Boyacá. Colombia 3Universidad de Jaén, Departamento de Biología Experimental, Jaén. España 4Universite de Montpellier I, Institut de Recherche pour le Developpement, Montpellier, Herault. Francia 5Instituto Nacional de Laboratorios de Salud, Laboratorio de Entomología Médica, La Paz. Bolivia 6Pontificia Universidad Católica del Ecuador, Facultad de Ciencias Exactas y Naturales, Quito, Pichincha. Ecuador 7Fundacao Oswaldo Cruz, Instituto Oswaldo Cruz, Rio de Janeiro. Brasil 8Universidad de la República, Centro Universitario Regional, Salto. Uruguay |
| Año: | 2021 |
| Volumen: | 116 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, analítico |
| Resumen en inglés | BACKGROUND Panstrongylus rufotuberculatus (Hemiptera-Reduviidae) is a triatomine species with a wide geographic distribution and a broad phenotypic variability. In some countries, this species is found infesting and colonising domiciliary ecotopes representing an epidemiological risk factor as a vector of Trypanosoma cruzi, etiological agent of Chagas disease. In spite of this, little is known about P. rufotuberculatus genetic diversity. METHODS Cytogenetic studies and DNA sequence analyses of one nuclear (ITS-2) and two mitochondrial DNA sequences (cyt b and coI) were carried out in P. rufotuberculatus individuals collected in Bolivia, Colombia, Ecuador and Mexico. Moreover, a geometric morphometrics study was applied to Bolivian, Colombian, Ecuadorian and French Guiana samples. OBJECTIVES To explore the genetic and phenetic diversity of P. rufotuberculatus from different countries, combining chromosomal studies, DNA sequence analyses and geometric morphometric comparisons. FINDINGS We found two chromosomal groups differentiated by the number of X chromosomes and the chromosomal position of the ribosomal DNA clusters. In concordance, two main morphometric profiles were detected, clearly separating the Bolivian sample from the other ones. Phylogenetic DNA analyses showed that both chromosomal groups were closely related to each other and clearly separated from the remaining Panstrongylus species. High nucleotide divergence of cyt b and coI fragments were observed among P. rufotuberculatus samples from Bolivia, Colombia, Ecuador and Mexico (Kimura 2-parameter distances higher than 9%). MAIN CONCLUSIONS Chromosomal and molecular analyses supported that the two chromosomal groups could represent different closely related species. We propose that Bolivian individuals constitute a new Panstrongylus species, being necessary a detailed morphological study for its formal description. The clear morphometric discrimination based on the wing venation pattern |
| Disciplinas: | Biología |
| Palabras clave: | Insectos, Evolución y filogenia, Enfermedad de Chagas, Vectores biológicos, Panstrongylus rufotuberculatus, Hemiptera, Especiación, Fusión cromosómica, Espaciadores intergénicos, Citocromo C oxidasa, Análisis morfométrico |
| Keyword: | Insects, Evolution and phylogeny, Chagas disease, Biological vectors, Panstrongylus rufotuberculatus, Hemiptera, Speciation, Chromosome fusion, Intergenic spacers, Cytochrome C oxidase, Morphometric analysis |
| Texte intégral: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
