| Revue: | Memorias do Instituto Oswaldo Cruz |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000459447 |
| ISSN: | 0074-0276 |
| Autores: | Prado, Tatiana1 Magalhães, Maithê Gaspar Pontes2 Moreira, Daniel Andrade2 Brandão, Martha Lima3 Fumian, Tulio Machado4 Ferreira, Fernando Cesar4 Chame, Marcia5 Leomil, Luciana6 Degrave, Wim Maurits Sylvain2 Leite, José Paulo Gagliardi4 Miagostovich, Marize Pereira4 |
| Instituciones: | 1Fundacao Oswaldo Cruz, Laboratorio de Vírus Respiratorios, Exantematicos, Enterovírus e Emergencias Virais, Rio de Janeiro. Brasil 2Fundacao Oswaldo Cruz, Laboratorio de Genomica Aplicada e BioInovacoes, Rio de Janeiro. Brasil 3Fundacao Oswaldo Cruz, Projeto FioAntar/VPPIS, Rio de Janeiro. Brasil 4Fundacao Oswaldo Cruz, Laboratorio de Virologia Comparada e Ambiental, Rio de Janeiro. Brasil 5Fundacao Oswaldo Cruz, Plataforma Institucional para Biodiversidade e Saude Animal, Rio de Janeiro. Brasil 6Servico Nacional de Aprendizagem Industrial, Centro Tecnologico para Industria Quimica e Textil, Rio de Janeiro. Brasil |
| Año: | 2023 |
| Volumen: | 118 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Aplicado, descriptivo |
| Resumen en inglés | Few studies have focused on microbial diversity in indoor environments of ships, as well as the role of the microbiome and its ecological interconnections. In this study, we investigated the microbiome and virome present on the internal surfaces of a polar ship in different stages (beginning, during, and at the end) of the Brazilian Antarctic expedition in order to evaluate abundance of microorganisms in different periods. OBJECTIVES AND METHODS We used shotgun metagenomic analysis on pooled samples from sampling surfaces in the ship’s interior to track the microbial diversity. FINDINGS Considering the total fraction of the microbiome, the relative abundance of bacteria, eukaryotes, viruses, and archaea was 83.7%, 16.2%, 0.04%, and 0.002%, respectively. Proteobacteria was the most abundant bacterial phyla, followed by Firmicutes, Actinobacteria, and Bacteroidetes. Concerning the virome, the greatest richness of viral species was identified during the middle of the trip, including ten viral families after de novo assembly: Autographiviridae, Chrysoviridae, Genomoviridae, Herelleviridae, Myoviridae, Partitiviridae, Podoviridae, Potyviridae, Siphoviridae, and Virgaviridae. MAIN CONCLUSIONS This study contributed to the knowledge of microbial diversity in naval transportation facilities, and variations in the abundance of microorganisms probably occurred due to factors such as the number of passengers and activities on the ship |
| Disciplinas: | Biología |
| Palabras clave: | Microbiología, Microbioma, Viroma, Metagenómica, Antártida |
| Keyword: | Microbiology, Antarctica, Metagenomics, Microbiome, Virome |
| Texte intégral: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
