| Revue: | Memorias do Instituto Oswaldo Cruz |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000459465 |
| ISSN: | 0074-0276 |
| Autores: | Campos, Hevandro de Souza1 Silva, Edimilson Domingos da2 Lima, Gerson Silva de2 Resende, Rafael de Oliveira3 Burth, Patricia1 |
| Instituciones: | 1Universidade Federal Fluminense, Departamento de Biologia Celular e Molecular, Niteroi, Rio de Janeiro. Brasil 2Fundacao Oswaldo Cruz, Bio-Manguinhos, Rio de Janeiro. Brasil 3Fundacao Oswaldo Cruz, Instituto Oswaldo Cruz, Rio de Janeiro. Brasil |
| Año: | 2023 |
| Volumen: | 118 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | BACKGROUND Leptospirosis is an emerging zoonosis that affects humans and animals. Immunochromatography rapid test is widely used for early diagnosis of leptospirosis, but with low sensitivity and specificity. OBJECTIVES To evaluate Leptospira interrogans insoluble fraction as a potential antigen source for lateral flow immunochromatography. METHODS Insoluble fraction derived from the crude bacterial extract was obtained by serial centrifugation. The polypeptide profile was determined using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Immune reactivity of this fraction was assessed by Western Blotting and lateral flow immunochromatography (LFI). It was tested 160 microagglutination test (MAT)-positive sera from patients in the acute phase, 100 MAT-negative sera from patients with acute febrile illness, and 45 patients with other infectious diseases. FINDINGS There was a predominance of low molecular mass-polypeptide bands, ranging from 2 to 37 kDa. The antibody reactivity of theses polypeptides was found to range from 13-50%, especially between 10 and 38 kDa. Among MAT-positive sera of patients with leptospirosis in the acute phase, 97% were also positive in LFI, indicating high sensitivity. Among MAT-negative sera, all were negative in LFI, indicating high specificity. Only 2% of cross-reactivity was detected. CONCLUSION The insoluble fraction can be a valuable antigen source for development of point-of-care diagnosis test for leptospirosis |
| Disciplinas: | Medicina |
| Palabras clave: | Diagnóstico, Bioquímica, Antigenos, Inmunocromatografía, Leptospirosis, Leptospira interrogans |
| Keyword: | Diagnosis, Biochemistry, Antigens, Leptospirosis, Immunochromatography, Leptospira interrogans |
| Texte intégral: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
