| Revue: | Memorias do Instituto Oswaldo Cruz |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000459459 |
| ISSN: | 0074-0276 |
| Autores: | Rubio Palis, Yasmin1 Dzuris, Nicole2 Sandi, Christopher2 Vizcaino Cabarrus, Rita Lucrecia2 Corredor Medina, Claudia2 González, Jesús Alberto3 Lenhart, Audrey E2 |
| Instituciones: | 1Universidad de Carabobo, Instituto de Investigaciones Biomédicas, Maracay, Aragua. Venezuela 2Centers for Disease Control and Prevention, Center for Global Health, Atlanta, Georgia. Estados Unidos de América 3Ministerio del Poder Popular para la Salud, Dirección General de Salud Ambiental, Maracay, Aragua. Venezuela |
| Año: | 2023 |
| Volumen: | 118 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | BACKGROUND The massive use of insecticides in public health has exerted selective pressure resulting in the development of resistance in Aedes aegypti to different insecticides in Venezuela. Between 2010 and 2020, the only insecticides available for vector control were the organophosphates (Ops) fenitrothion and temephos which were focally applied. OBJECTIVES To determine the state of insecticide resistance and to identify the possible biochemical and molecular mechanisms involved in three populations of Ae. aegypti from Venezuela. METHODS CDC bottle bioassays were conducted on Ae. aegypti collected between October 2019 and February 2020 in two hyperendemic localities for dengue in Aragua State and in a malaria endemic area in Bolívar State. Insecticide resistance mechanisms were studied using biochemical assays and polymerase chain reaction (PCR) to detect kdr mutations. FINDINGS Bioassays showed contrasting results among populations; Las Brisas was resistant to malathion, permethrin and deltamethrin, Urbanización 19 de Abril was resistant to permethrin and Nacupay to malathion. All populations showed significantly higher activity of mixed function oxidases and glutathione-S-transferases (GSTs) in comparison with the susceptible strain. The kdr mutations V410L, F1534C, and V1016I were detected in all populations, with F1534C at higher frequencies. MAIN CONCLUSION Insecticide resistance persists in three Ae. aegypti populations from Venezuela even in the relative absence of insecticide application |
| Disciplinas: | Biología |
| Palabras clave: | Insectos, Fisiología animal, Mosquitos, Resistencia a insecticidas, Mutaciones génicas, Destoxificacion metabólica, Aedes aegypti |
| Keyword: | Insects, Animal physiology, Mosquitoes, Insecticides resistance, Metabolic detoxification, Gene mutations, Aedes aegypti |
| Texte intégral: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
