| Revue: | Memorias do Instituto Oswaldo Cruz |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000459468 |
| ISSN: | 0074-0276 |
| Autores: | Chaves-Filho, Adriano José Maia1 Jucá, Paloma Marinho1 Soares, Michelle Verde Ramo1 Oliveira, Caio Andrade de1 Sousa, Raul Cavalcante de1 Lós, Deniele Bezerra1 Russo, Remo Castro2 Yaochite, Juliana Navarro Ueda3 Macedo, Danielle S1 |
| Instituciones: | 1Universidade Federal do Ceara, Faculdade de Medicina, Fortaleza, Ceara. Brasil 2Universidade Federal de Minas Gerais, Instituto de Ciencias Biologicas, Belo Horizonte, Minas Gerais. Brasil 3Universidade Federal do Ceara, Faculdade de Farmacia, Odontologia e Enfermagem, Fortaleza, Ceara. Brasil |
| Año: | 2023 |
| Volumen: | 118 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | BACKGROUND The novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants can infect common mice inducing significant pathological lung lesions and inflammatory responses. This substantially mimics coronavirus disease 19 (COVID-19) infection and pathogenesis in humans. OBJECTIVES To characterise the effects of recombinant SARS-CoV-2 S1 receptor-binding domain (RBD) peptide in murine macrophage and microglial cells’ immune activation compared with classical PAMPs in vitro. METHODS Murine RAW 264.7 macrophages and BV2 microglial cells were exposed to increasing concentrations of the RBD peptide (0.01, 0.05, and 0.1 µg/mL), Lipopolysaccharide (LPS) and Poly(I:C) and evaluated after two and 24 h for significant markers of macrophage activation. We determined the effects of RBD peptide on cell viability, cleaved caspase 3 expressions, and nuclear morphometry analysis. FINDINGS In RAW cells, RBD peptide was cytotoxic, but not for BV2 cells. RAW cells presented increased arginase activity and IL-10 production; however, BV2 cells expressed iNOS and IL-6 after RBD peptide exposure. In addition, RAW cells increased cleaved-caspase-3, apoptosis, and mitotic catastrophe after RBD peptide stimulation but not BV2 cells. CONCLUSION RBD peptide exposure has different effects depending on the cell line, exposure time, and concentration. This study brings new evidence about the immunogenic profile of RBD in macrophage and microglial cells, advancing the understanding of SARS-Cov2 immuno- and neuropathology |
| Disciplinas: | Medicina |
| Palabras clave: | Virus, Inmunología, SARS-CoV-2, COVID-19, Macrófagos, Microglía, Neuroinflamación, Inmunidad innata |
| Keyword: | Virus, Immunology, COVID-19, SARS-CoV-2, Macrophages, Microglia, Neuroinflammation, Innate immunity |
| Texte intégral: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
