| Revista: | Memorias do Instituto Oswaldo Cruz |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000393391 |
| ISSN: | 0074-0276 |
| Autores: | Ximenes, Camila1 Brandao, Eduardo1 Oliveira, Paula1 Rocha, Abraham1 Rego, Tamisa1 Medeiros, Rafael1 Aguiar-Santos, Ana1 Ferraz, Joao1 Reis, Christian2 Araujo, Paulo1 Carvalho, Luiz3 Melo, Fabio L1 |
| Instituciones: | 1Centro de Pesquisas Aggeu Magalhaes, Servico de Referencia Nacional em Filarioses, Recife, Pernambuco. Brasil 2Centro de Pesquisas Aggeu Magalhaes, Departamento de Microbiologia, Recife, Pernambuco. Brasil 3Universidade Federal de Pernambuco, Laboratorio de Imunopatologia Keizo Asami, Recife, Pernambuco. Brasil |
| Año: | 2014 |
| Periodo: | Dic |
| Volumen: | 109 |
| Número: | 8 |
| Paginación: | 978-983 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | The Global Program for the Elimination of Lymphatic Filariasis (GPELF) aims to eliminate this disease by the year 2020. However, the development of more specific and sensitive tests is important for the success of the GPELF. The present study aimed to standardise polymerase chain reaction (PCR)-based systems for the diagnosis of filariasis in serum and urine. Twenty paired biological urine and serum samples from individuals already known to be positive for Wuchereria bancrofti were collected during the day. Conventional PCR and semi-nested PCR assays were optimised. The detection limit of the technique for purified W. bancrofti DNA extracted from adult worms was 10 fg for the internal systems (WbF/Wb2) and 0.1 fg by using semi-nested PCR. The specificity of the primers was confirmed experimentally by amplification of 1 ng of purified genomic DNA from other species of parasites. Evaluation of the paired urine and serum samples by the semi-nested PCR technique indicated only two of the 20 tested individuals were positive, whereas the simple internal PCR system (WbF/Wb2), which has highly promising performance, revealed that all the patients were positive using both samples. This study successfully demonstrated the possibility of using the PCR technique on urine for the diagnosis of W. bancrofti infection |
| Disciplinas: | Medicina |
| Palabras clave: | Diagnóstico, Parasitología, Filariasis linfática, Técnicas de diagnóstico, ADN, Wuchereria bancrofti, Orina, Suero humano, Reacción en cadena de la polimerasa (PCR) |
| Keyword: | Medicine, Diagnosis, Parasitology, Lymphatic filariasis, Diagnostic techniques, DNA, Wuchereria bancrofti, Urine, Human serum, Polymerase chain reaction (PCR) |
| Texto completo: | Texto completo (Ver HTML) |
