| Revue: | Memorias do Instituto Oswaldo Cruz |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000459436 |
| ISSN: | 0074-0276 |
| Autores: | Gonçalves, Marina Neves1 Lopes, Daiana Silva1 Teixeira, Samuel Cota3 Teixeira, Thaise Lara4 de Freitas, Vitor1 Costa, Tássia Rafaella1 Gimenes, Sarah Natalie Cirilo5 de Camargo, Isabella Mitie5 de Souza, Guilherme3 da Silva, Marcelo Santos6 Azevedo, Fernanda Van Petten de Vasconcelos1 Grego, Kathleen Fernandes7 Santos, Luísa Carregosa2 Oliveira, Vinícius Queiroz2 da Silva, Claudio Vieira3 Rodrigues, Renata Santos1 Yoneyama, Kelly Aparecida Geraldo1 Clissa, Patricia Bianca5 Rodrigues, Veridiana de Melo1 |
| Instituciones: | 1Universidade Federal de Uberlandia, Instituto de Biotecnologia, Uberlandia, Minas Gerais. Brasil 2Universidade Federal da Bahia, Instituto de Biociencias, Vitoria da Conquista, Bahia. Brasil 3Universidade Federal de Uberlandia, Instituto de Ciencias Biomedicas, Uberlandia, Minas Gerais. Brasil 4Universidade Federal de Sao Paulo, Escola Paulista de Medicina, Sao Paulo. Brasil 5Instituto Butantan, Laboratorio de Imunopatologia, Sao Paulo. Brasil 6Universidade de Sao Paulo, Instituto de Quimica, Sao Paulo. Brasil 7Instituto Butantan, Laboratório de Herpetologia, Sao Paulo. Brasil |
| Año: | 2023 |
| Volumen: | 118 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | BACKGROUND Leishmaniasis, a neglected disease caused by the parasite Leishmania, is treated with drugs associated with high toxicity and limited efficacy, in addition to constant reports of the emergence of resistant parasites. In this context, snake serums emerge as good candidates since they are natural sources with the potential to yield novel drugs. OBJECTIVES We aimed to show the antileishmanial effects of γCdcPLI, a phospholipase A2 inhibitor from Crotalus durissus collilineatus snake serum, against Leishmania (Leishmania) amazonensis. METHODS Promastigotes forms were exposed to γCdcPLI, and we assessed the parasite viability and cell cycle, as well as invasion and proliferation assays. FINDINGS Despite the low cytotoxicity effect on macrophages, our data indicate that γCdcPLI has a direct effect on parasites promoting an arrest in the G1 phase and reduction in the G2/M phase at the highest dose tested. Moreover, this PLA2 inhibitor reduced the parasite infectivity when promastigotes were pre-treated. Also, we demonstrated that the γCdcPLI treatment modulated the host cell environment impairing early and late steps of the parasitism. MAIN CONCLUSIONS γCdcPLI is an interesting tool for the discovery of new essential targets on the parasite, as well as an alternative compound to improve the effectiveness of the leishmaniasis treatment |
| Disciplinas: | Medicina, Química |
| Palabras clave: | Química farmacéutica, Suero, Veneno de víbora, Inhibidores enzimáticos, Fosfolipasa A2, Actividad biológica, Leishmaniasis, Crotalus durissus collilineatus |
| Keyword: | Medicinal chemistry, Snake venom, Serum, Enzyme inhibitors, Phospholipase A2, Biological activity, Crotalus durissus collilineatus, Leishmaniasis |
| Texte intégral: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
