| Revue: | Maderas : ciencia y tecnología |
| Base de datos: | |
| Número de sistema: | 000544590 |
| ISSN: | 0718-221X |
| Autores: | Karami, Leila1 Fromm, Jörg1 Koch, Gerald1 Schmidt, Olaf1 Schmitt, Uwe1 |
| Instituciones: | 1University of Hamburg, Section Wood Biology, Hamburg. Alemania |
| Año: | 2014 |
| Periodo: | May |
| Volumen: | 16 |
| Número: | 2 |
| Paginación: | 149-158 |
| País: | Chile |
| Idioma: | Inglés |
| Resumen en inglés | Decayed-wood samples were collected from a naturally-infected bridge made of Quercus robur. Fruiting bodies of the white-rot basidiomycetes Hymenochaete rubiginosa and Stereum hirsutum were sampled. The white-rot fungus Fuscoporia ferrea and the secondary saprobiont Mycena galericulata were identified from the rotten wood by means of rDNA-ITS sequencing. The topochemistry of lignin degradation within individual cell-wall layers was determined by cellular UV-microspectrophotometry (UMSP) at 278 nm wavelength. Increased delignification occurred in compound middle lamella regions (CML), secondary wall layers of fibres (S2), longitudinal and ray parenchyma cells as well in vessels. The highest lignin content at initial and medium decay was recorded in the CML, whereas in advanced decay secondary wall layers of the vessels exhibited the highest content of residual lignin. In all stages of degradation, the S2 layers of fibres had the lowest lignin amount. |
| Keyword: | Oak wood degradation, Fungi, RDNA-ITS sequencing, Lignin content, UV-microspectrophotometry |
| Texte intégral: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
