Protective role of exosomes derived from regulatory T cells against inflammation and apoptosis of BV-2 microglia under oxygen-glucose deprivation/reperfusion challenge
Título del documento: Protective role of exosomes derived from regulatory T cells against inflammation and apoptosis of BV-2 microglia under oxygen-glucose deprivation/reperfusion challenge
Revue: Genetics and molecular biology
Base de datos: PERIÓDICA
Número de sistema: 000459277
ISSN: 1415-4757
Autores: 1
3
1
1
4
2
Instituciones: 1The Third Affiliated Hospital of Luohe Medical College, Department of Rehabilitation, Luohe, Henan. China
2Luohe Medical College, Department of Medicine, Luohe, Henan. China
3Luohe Hospital of Traditional Chinese Medicine, Department of Emergency, Luohe, Henan. China
4Luohe Medical College, Office of Research Management, Luohe, Henan. China
Año:
Volumen: 45
Número: 4
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, analítico
Resumen en inglés Regulatory T cells (Tregs) are found to participate in the pathogenesis of cerebral ischemic stroke. Exosomes derived from Tregs (Treg-Exos) were found to mediate the mechanism of Tregs’ roles under various physiological and pathological conditions. But the roles of Treg-Exos in cerebral ischemic stroke are still unclear. Here, we explored the protective effects of Treg-Exos against microglial injury in response to oxygen-glucose deprivation/reperfusion (OGD/R) exposure. The results showed that Tregs-Exos relieved OGD/R-caused increases in LDH release and caspase-3 activity in BV-2 cells. The decreased cell viability and increased percentage of TUNEL-positive cells in OGD/R-exposed BV-2 cells were attenuated by Tregs-Exos treatment. Tregs-Exos also suppressed OGD/R-induced increase in production of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 in BV-2 microglia. Furthermore, Tregs-Exos induced the expression levels of phosphorylated phosphatidylinositol-3-kinase (p-PI3K) and phosphorylated protein kinase B (p-Akt) in BV-2 microglia under the challenge of OGD/R. Inhibition of the PI3K/Akt signaling by LY294002 partly reversed the effects of Tregs-Exos on cell apoptosis and inflammation in OGD/R-exposed BV-2 microglia. These results indicated that Tregs-Exos exerted protective effects against the OGD/R-caused injury of BV-2 microglia by activating the PI3K/Akt signaling
Disciplinas: Medicina
Palabras clave: Medicina experimental,
Neurología,
Células T,
Inflamación,
Apoptosis,
Microglía,
Exosomas,
Infarto cerebral
Keyword: Experimental medicine,
Neurology,
T cells,
Inflammation,
Apoptosis,
Exosomes,
Ischemic stroke,
Microglia
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