| Revue: | Genetics and molecular biology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000424656 |
| ISSN: | 1415-4757 |
| Autores: | Oliveira, Eder Silva de1 Junges, Angela1 Sbaraini, Nicolau1 Andreis, Fábio Carrer1 Thompson, Claudia Elizabeth1 Staats, Charley Christian1 Schrank, Augusto1 |
| Instituciones: | 1Universidade Federal do Rio Grande do Sul, Centro de Biotecnologia, Porto Alegre, Rio Grande do Sul. Brasil |
| Año: | 2018 |
| Periodo: | Dic |
| Volumen: | 41 |
| Número: | 4 |
| Paginación: | 843-857 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Analítico, descriptivo |
| Resumen en inglés | AbstractCell walls are involved in manifold aspects of fungi maintenance. For several fungi, chitin synthesis, degradation andrecycling are essential processes required for cell wall biogenesis; notably, the activity ofb-N-acetylglucosa-minidases (NAGases) must be present for chitin utilization. For entomopathogenic fungi, such asMetarhiziumanisopliae, chitin degradation is also used to breach the host cuticle during infection. In view of the putative role ofNAGases as virulence factors, this study explored the transcriptional profile and evolution of putative GH20NAGases (MaNAG1andMaNAG2) and GH3 NAGases (MaNAG3andMaNAG4) identified inM. anisopliae. WhileMaNAG2 orthologs are conserved in several ascomycetes, MaNAG1 clusters only withAspergilllussp. andentomopathogenic fungal species. By contrast, MaNAG3 and MaNAG4 were phylogenetically related with bacterialGH3 NAGases. The transcriptional profiles ofM. anisopliaeNAGase genes were evaluated in seven culture condi-tions showing no common regulatory patterns, suggesting that these enzymes may have specific roles during theMetarhiziumlife cycle. Moreover, the expression ofMaNAG3andMaNAG4regulated by chitinous substrates is thefirst evidence of the involvement of putative GH3 NAGases in physiological cell processes in entomopathogens, indi-cating their potential influence on cell differentiation during theM. anisopliaelife cycle |
| Disciplinas: | Biología, Química |
| Palabras clave: | Genética, Evolución y filogenia, Bioquímica, Gen NAGase, Gen GH20 NaGase, Gen GH3 NaGase, Metarhizium anisopliae, Sistema quitinolítico, Hongos, Entomopatógenos |
| Keyword: | Genetics, Evolution and phylogeny, Biochemistry, NAGase gene, GH20 and GH3, Metarhizium anisopliae, Chitinolytic system, Fungi, Entomopathogens |
| Texte intégral: | Texto completo (Ver PDF) |
