Low STR variability in the threatened marsh deer, Blastocerus dichotomus, detected through amplicon sequencing in non-invasive samples
Título del documento: Low STR variability in the threatened marsh deer, Blastocerus dichotomus, detected through amplicon sequencing in non-invasive samples
Revue: Genetics and molecular biology
Base de datos: PERIÓDICA
Número de sistema: 000459271
ISSN: 1415-4757
Autores: 1
2
2
1
3
Instituciones: 1Museo Argentino de Ciencias Naturales "Bernardino Rivadavia", División de Mastozoología, Buenos Aires. Argentina
2Dalhousie University, Department of Biology, Halifax, Nueva Escocia. Canadá
3Universidade Federal do Rio de Janeiro, Instituto de Biologia, Rio de Janeiro. Brasil
Año:
Volumen: 45
Número: 4
País: Brasil
Idioma: Inglés
Tipo de documento: Nota breve o noticia
Enfoque: Experimental, analítico
Resumen en inglés Blastocerus dichotomus is the largest deer in South America. We have used 25 microsatellite markers detected and genotyped by Next Generation Sequencing to estimate the genetic variability of B. dichotomus in Argentina, where most of its populations are threatened. Primer design was based on the sequence of a shallow partial genome (15,967,456 reads; 16.66% genome coverage, mean depth 1.64) of a single individual. From the thousands of microsatellite loci found, even under high stringency selection, we chose and tested a set of 80 markers on 30 DNA samples extracted from tissue and feces from three Argentinean populations. Heterozygosity levels were low across all loci in all populations (H=0.31 to 0.40). Amplicon sequencing is a fast, easy, and affordable technique that can be very useful for the characterization of microsatellite marker sets for the conservation genetics of non-model organisms. This work is also one of the first ones to use amplicon sequencing in non-invasive samples and represents an important development for the study of threatened species
Disciplinas: Biología
Palabras clave: Genética,
Vertebrados,
Mamíferos,
Blastocerus dichotomus,
Ciervos,
Microsatélites,
Secuenciación de próxima generación (NGS)
Keyword: Genetics,
Vertebrates,
Mammals,
Blastocerus dichotomus,
Deers,
Next Generation Sequencing (NGS),
Microsatellites
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