| Revue: | Genetics and molecular biology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000424677 |
| ISSN: | 1415-4757 |
| Autores: | Pereira, Mayra Ramos de Jesus1 Pinhatti, Valéria Rodrigues1 Silveira, Maiele Dornelles da1 Matzenbacher, Cristina Araujo2 Freitas, Thales Renato Ochotorena de2 Silva, Juliana da1 Camassola, Melissa1 Nardi, Nance Beyer1 |
| Instituciones: | 1Universidade Luterana do Brasil, Laboratório de Células-Tronco e Engenharia de Tecidos, Canoas, Rio Grande do Sul. Brasil 2Universidade Federal do Rio Grande do Sul, Departamento de Genetica, Porto Alegre, Rio Grande do Sul. Brasil |
| Año: | 2018 |
| Periodo: | Dic |
| Volumen: | 41 |
| Número: | 4 |
| Paginación: | 870-877 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental |
| Resumen en inglés | Abstract Mesenchymal stem/stromal cells (MSCs) are multipotent cells distributed in all tissues and characterized by adherence, morphology, immunophenotype and trilineage differentiation potential. The present study aimed to isolate and characterize adherent MSC-like populations from different tissues of Ctenomys minutus, a threatened wildlife rodent popularly known as tuco-tuco. Adherent cells were isolated from bone marrow, brain, liver, pancreas and adipose tissue of three adult animals collect in southern Brazil. Cultures showed typical morphology and proliferation potential.Adipose-derived MSCs showed trilineage potential. Cultures derived from adipose tissue, bone marrow and brain were immunophenotyped with negative results for CD31, CD44, CD45, CD106, and MHC class II, as well as strongpositive results for CD29. Low fluorescence levels were seen for CD49d, CD90.2 and CD117. Cultures were negative for CD49e, except for brain-derived cultures that were weakly positive. CD11b was negative in adipose-derivedMSCs, but positive in brain and bone marrow-derived cultures. The scratch assay showed high migration potentialfor pancreas and adipose tissue-derived cells. This study represents the first report of isolation and characterization of cultures having characteristics of MSCs from Ctenomys minutus. The collection of biological information forbiobanks represents an important contribution to the creation of strategies for prevention of loss of genetic diversity |
| Disciplinas: | Biología |
| Palabras clave: | Biología celular, Anatomía e histología, Genética, Células mesenquimales del tallo/ estroma, Tuco-tuco, Ctenomys minutus |
| Keyword: | Cell biology, Anatomy and histology, Genetics, Mesenchymal stem/stromal cells, Tuco-tuco, Ctenomys minutus |
| Texte intégral: | Texto completo (Ver PDF) |
