| Revue: | Genetics and molecular biology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000418598 |
| ISSN: | 1415-4757 |
| Autores: | Zhen Gong1 Jianyun Liu2 Xin Xie2 Xiaoyuan Xu2 Ping Wu2 Huimin Li1 Yagin Wang3 Weidong Li2 Jianjun Xiong1 |
| Instituciones: | 1Jiujiang University, College of Basic Medical Science, Jiujiang, Jiangxi. China 2Jiujiang University, Key Laboratory of Jiangxi Province for the Systemic Bio-medicine, Jiujiang, Jiangxi. China 3Wuhan University, Renmin Hospital, Wuhan, Hubei. China |
| Año: | 2018 |
| Periodo: | Jun |
| Volumen: | 41 |
| Número: | 2 |
| Paginación: | 488-495 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Analítico |
| Resumen en inglés | The ubiquitin-specific protease 22 (USP22) is an oncogene and its expression is upregulated in many types of cancer. In the nucleus, USP22 functions as one subunit of the SAGA to regulate gene transcription. However, the genome-wide USP22 binding sites and its direct target genes are yet clear. In this study, we characterized the potential genomic binding sites of UPS22 and GCN5 by ChIP-seq using specific antibodies in HeLa cells. There were 408 overlapping putative target genes bound by both USP22 and GCN5. Motif analysis showed that the sequences bound by USP22 and GCN5 shared two common motifs. Gene ontology (GO) and pathway analysis indicated that the genes targeted by USP22 and GCN5 were involved in different physiological processes and pathways. Further RNA-seq, GO and pathway analyses revealed that knockdown of UPS22 induced differential expression of many genes that participated in diverse physiological processes, such as metabolic process. Integration of ChIP-seq and RNA-seq data revealed that UPS22 bound to the promoters of 56 genes. These findings may provide new insights into the regulation of USP22 on gene expression during the development of cervical cancer |
| Disciplinas: | Química, Biología |
| Palabras clave: | Bioquímica, Genética, Biología celular, USP22, Genes diana, ChIP-seq, Gen knockdown, RNA-Seq |
| Keyword: | Biochemistry, Genetics, Cell biology, USP22, Target genes, ChIP-seq, Knockdown gene, RNA-Seq |
| Texte intégral: | Texto completo (Ver PDF) |
