Functional characterization of sugarcane mustang domesticated transposases and comparative diversity in sugarcane, rice, maize and sorghum
Título del documento: Functional characterization of sugarcane mustang domesticated transposases and comparative diversity in sugarcane, rice, maize and sorghum
Revue: Genetics and molecular biology
Base de datos: PERIÓDICA
Número de sistema: 000351295
ISSN: 1415-4757
Autores: 1
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Instituciones: 1Universidade de Sao Paulo, Instituto de Biociencias, Sao Paulo. Brasil
Año:
Volumen: 35
Número: 3
Paginación: 632-639
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés Transposable elements (TEs) account for a large portion of plant genomes, particularly in grasses, in which they correspond to 50%-80% of the genomic content. TEs have recently been shown to be a source of new genes and new regulatory networks. The most striking contribution of TEs is referred as “molecular domestication”, by which the element coding sequence loses its movement capacity and acquires cellular function. Recently, domesticated transposases known as mustang and derived from the Mutator element have been described in sugarcane. In order to improve our understanding of the function of these proteins, we identified mustang genes from Sorghum bicolor and Zea mays and performed a phenetic analysis to assess the diversity and evolutionary history of this gene family. This analysis identified orthologous groups and showed that mustang genes are highly conserved in grass genomes. We also explored the transcriptional activity of sugarcane mustang genes in heterologous and homologous systems. These genes were found to be ubiquitously transcribed, with shoot apical meristem having the highest expression levels, and were downregulated by phytohormones. Together, these findings suggest the possible involvement of mustang proteins in the maintenance of hormonal homeostasis
Disciplinas: Biología
Palabras clave: Genética,
Gramíneas,
Caña de azúcar,
Elementos transponibles,
Diversidad genética,
Analisis fenético,
Arroz,
Maíz,
Sorgo
Keyword: Biology,
Genetics,
Gramineae,
Sugar cane,
Transposable elements,
Genetic diversity,
Phenetic analysis,
Rice,
Corn,
Sorghum
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