Evolution of DNMT2 in drosophilids: Evidence for positive and purifying selection and insights into new protein (pathways) interactions



Título del documento: Evolution of DNMT2 in drosophilids: Evidence for positive and purifying selection and insights into new protein (pathways) interactions
Revue: Genetics and molecular biology
Base de datos: PERIÓDICA
Número de sistema: 000418067
ISSN: 1415-4757
Autores: 1
2
3
4
4
Instituciones: 1Universidade Federal do Rio Grande do Sul, Instituto de Biociencias, Porto Alegre, Rio Grande do Sul. Brasil
2Universidade Federal de Santa Maria, Departamento de Zoologia e Ciencias Biologicas, Palmeira das Missoes, Rio Grande do Sul. Brasil
3Universidade Federal do Rio Grande do Sul, Programa de Pos-Graduacao em Genetica e Biologia Molecular, Porto Alegre, Rio Grande do Sul. Brasil
4Universidade Federal do Rio Grande do Sul, Programa de Pos-Graduacao em Biologia Animal, Porto Alegre, Rio Grande do Sul. Brasil
Año:
Periodo: Mar
Volumen: 41
Paginación: 215-234
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Analítico
Resumen en inglés The DNA methyltransferase 2 (DNMT2) protein is the most conserved member of the DNA methyltransferase family. Nevertheless, its substrate specificity is still controversial and elusive. The genomic role and determinants of DNA methylation are poorly understood in invertebrates, and several mechanisms and associations are suggested. In Drosophila, the only known DNMT gene is Dnmt2. Here we present our findings from a wide search for Dnmt2 homologs in 68 species of Drosophilidae. We investigated its molecular evolution, and in our phylogenetic analyses the main clades of Drosophilidae species were recovered. We tested whether the Dnmt2 has evolved neutrally or under positive selection along the subgenera Drosophila and Sophophora and investigated positive selection in relation to several physicochemical properties. Despite of a major selective constraint on Dnmt2, we detected six sites under positive selection. Regarding the DNMT2 protein, 12 sites under positive-destabilizing selection were found, which suggests a selection that favors structural and functional shifts in the protein. The search for new potential protein partners with DNMT2 revealed 15 proteins with high evolutionary rate covariation (ERC), indicating a plurality of DNMT2 functions in different pathways. These events might represent signs of molecular adaptation, with molecular peculiarities arising from the diversity of evolutionary histories experienced by drosophilids
Disciplinas: Biología,
Química
Palabras clave: Insectos,
Evolución y filogenia,
Bioquímica,
Drosophila,
Proteína Dnmt2,
Selección positiva,
Epigenética,
Selección desestabilizadora
Keyword: Insects,
Evolution and phylogeny,
Biochemistry,
Drosophila,
Dnmt2 protein,
Positive selection,
Epigenetics,
Destabilizing selection
Texte intégral: Texto completo (Ver PDF)