Revista: | Genetics and molecular biology |
Base de datos: | PERIÓDICA |
Número de sistema: | 000356573 |
ISSN: | 1415-4757 |
Autores: | Rizo, Walace Fraga1 Ferreira, Luis Eduardo1 Colnaghi, Vanessa1 Martins, Juliana Simoes1 Franchi, Leonardo Pereira2 Takahashi, Catarina Satie2 Beleboni, Rene Oliveira1 Marins, Mozart1 Pereira, Paulo Sergio1 Fachin, Ana Lucia1 |
Instituciones: | 1Universidade de Ribeirao Preto, Unidade de Biotecnologia Vegetal, Ribeirao Preto, Sao Paulo. Brasil 2Universidade de Sao Paulo, Faculdade de Medicina de Ribeirao Preto, Ribeirao Preto, Sao Paulo. Brasil |
Año: | 2013 |
Periodo: | Mar |
Volumen: | 36 |
Número: | 1 |
Paginación: | 105-110 |
País: | Brasil |
Idioma: | Inglés |
Tipo de documento: | Artículo |
Enfoque: | Experimental, aplicado |
Resumen en inglés | Cancer has become a major public health problem worldwide and the number of deaths due to this disease is increasing almost exponentially. In the constant search for new treatments, natural products of plant origin have provided a variety of new compounds to be explored as antitumor agents. Tabernaemontana catharinensis is a medicinal plant that produces alkaloids with expressive antitumor activity, such as heyneanine, coronaridine and voacangine. The aim of present study was firstly to screen the cytotoxic activity of the indole alkaloids heyneanine, coronaridine and voacangine against HeLa (human cervix tumor), 3T3 (normal mouse embryo fibroblasts), Hep-2 (human laryngeal epithelial carcinoma) and B-16 (murine skin) cell lines by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide); and secondly to analyze the apoptotic activity, cell membrane damage and genotoxicity of the compound that showed the best cytotoxic activity against the tumor cell lines tested. Coronaridine was the one that exhibited greater cytotoxic activity in the laryngeal carcinoma cell line Hep-2 (IC50 = 54.47 µg/mL) than the other alkaloids tested (voacangine IC50 = 159.33 µg/mL, and heyneanine IC50 = 689.45 µg/mL). Coronaridine induced apoptosis in cell lines 3T3 and Hep-2, even at high concentrations. The evaluation of genotoxicity by comet assay showed further that coronaridine caused minimal DNA damage in the Hep-2 tumor cell line, and the LDH test showed that it did not affect the plasma membrane. These results suggest that further investigation of coronaridine as an antitumor agent has merit |
Disciplinas: | Biología, Medicina |
Palabras clave: | Oncología, Fitoquímica, Actividad citotóxica, Alcaloides indólicos, Genotoxicidad, Coronaridina, Carcinoma epitelial laríngeo, Apoptosis, Tabernaemontana catharinensis |
Keyword: | Biology, Medicine, Oncology, Phytochemistry, Cytotoxic activity, Genotoxicity, Coronaridine, Laryngeal epithelial carcinoma, Apoptosis, Tabernaemontana catharinensis |
Texto completo: | Texto completo (Ver HTML) |