| Revue: | Genetics and molecular biology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000417810 |
| ISSN: | 1415-4757 |
| Autores: | Grubisa, Ivana1 Otasevic, Petar2 Vucinic, Nada1 Milicic, Biljana3 Jozic, Tanja4 Krstic, Slobodan5 Milasin, Jelena6 |
| Instituciones: | 1University of Belgrade, Department of Human Genetics and Prenatal Diagnostics, Belgrado. Serbia 2Dedinje Cardiovascular Institute, Belgrado. Serbia 3University of Belgrade, Department of Statistics, Belgrado. Serbia 4Clinical Center of Serbia, Clinic of Cardiology, Belgrado. Serbia 5Clinical Center of Serbia, Emergency Center, Belgrado. Serbia 6University of Belgrade, Department of Human Genetics, Belgrado. Serbia |
| Año: | 2018 |
| Periodo: | Mar |
| Volumen: | 41 |
| Número: | 1 |
| Paginación: | 35-40 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Analítico |
| Resumen en inglés | Oxidative stress (OS) plays an important role in atherogenesis and since glutathione S-transferases (GSTs) provide protection against OS, we have tested the hypothesis that deletion polymorphisms in two GSTs (GSTM1 and GSTT1) may affect the risk of developing atherosclerosis. A total of 382 individuals (200 patients with atherosclerosis and 182 healthy controls) were included in this association study. Genomic DNA was isolated from peripheral blood cells or from buccal epithelial cells and genotyping was performed using multiplex-PCR or real-time PCR methods. GSTM1 null genotype was significantly more frequent in atherosclerotic patients than in controls (52.0% vs 34.1%) and individuals with the GSTM1 null genotype had an approximately 2-fold increase in atherosclerosis risk (OR: 2.1, 95%CI=1.39-3.17, P=0.0004). GSTT1 null genotype alone did not show a statistically significant effect on atherosclerosis risk modulation, but the association approached significance (OR: 1.57, 95%CI=0.94-2.64, P=0.08). The combined analysis showed that the presence of both genes had a protective effect against atherosclerosis (OR=0.55, 95%CI=0.37-0.83, P=0.005) while double null genotypes led to a robust atherosclerosis risk increase (OR: 8.14, 95%CI= 2.41-27.51, P < 0.0001). This study demonstrated that the GSTM1 null and combined GSTM1/GSTT1 null genotypes are susceptibility factors for development of atherosclerosis in a Serbian population |
| Disciplinas: | Medicina, Química |
| Palabras clave: | Sistema cardiovascular, Bioquímica, Genética, Aterosclerosis, Polimorfismo genético, Glutatión S-transferasa, Estrés oxidativo, Población Serbia |
| Keyword: | Cardiovascular system, Biochemistry, Genetics, Atherosclerosis, Genetic polymorphism, Glutathione S-transferase, Oxidative stress, Serbian population |
| Texte intégral: | Texto completo (Ver PDF) |
