Can plant DNA barcoding be implemented in species-rich tropical regions? A perspective from São Paulo State, Brazil



Título del documento: Can plant DNA barcoding be implemented in species-rich tropical regions? A perspective from São Paulo State, Brazil
Revue: Genetics and molecular biology
Base de datos: PERIÓDICA
Número de sistema: 000420043
ISSN: 1415-4757
Autores: 1
1
2
3
4
1
5
2
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5
Instituciones: 1Universidade de Sao Paulo, Instituto de Biociencias, Sao Paulo. Brasil
2Universidade de Sao Paulo, Escola Superior de Agricultura "Luiz de Queiroz", Piracicaba, Sao Paulo. Brasil
3Universidade Estadual de Campinas, Instituto de Biologia, Campinas, Sao Paulo. Brasil
4Universidade de Sao Paulo, Escola de Artes, Ciencias e Humanidades, Sao Paulo. Brasil
5Universite Paul Sabatier, Laboratoire Evolution et Diversité Biologique, Toulouse, Haute-Garonne. Francia
Año:
Periodo: Sep
Volumen: 41
Número: 3
Paginación: 661-670
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Analítico
Resumen en inglés DNA barcoding helps to identify species, especially when identification is based on parts of organisms or life stages such as seeds, pollen, wood, roots or juveniles. However, the implementation of this approach strongly depends on the existence of complete reference libraries of DNA sequences. If such a library is incomplete, DNA-based identification will be inefficient. Here, we assess if DNA barcoding can already be implemented in species-rich tropical regions. We focus on the tree flora of São Paulo state, Brazil, which contains more than 2000 tree species. Using new DNA sequence data and carefully assembled GenBank accessions, we assembled 12,113 sequences from ten different regions. The ITS, rbc L, psb A- trn H, mat K and trn L regions were better represented within the available se- quences for São Paulo tree flora. Currently, only 58% of the São Paulo tree flora currently have at least one barcoding sequence available. However, these species represent on average 89% of the trees in São Paulo state forests. Therefore, conservation-oriented and ecological studies can already benefit from DNA barcoding to obtain more accurate species identifications. We present which taxa remain underrepresented for the São Paulo tree flora and discuss the implications of this result for other species-rich tropical regions
Disciplinas: Biología
Palabras clave: Botánica,
Genética,
Ecología,
Evaluación,
Biodiversidad,
Código de barras,
Plantas,
Conservación de especies,
Especies de árboles
Keyword: Botany,
Genetics,
Ecology,
Assessment,
Biodiversity,
Plant barcoding,
Species conservation,
Tree species
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