| Revue: | Genetics and molecular biology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000459269 |
| ISSN: | 1415-4757 |
| Autores: | Linh, Nguyen Thi Thuy1 Giang, Nguyen Hoang2 Lien, Nguyen Thi Kim2 Trang, Bui Kieu2 Trang, Do Thi2 Ngoc, Nguyen Thy3 Nghia, Vu Xuan4 My, Le Tra2 Mao, Can Van5 Hoang, Nguyen Huy1 Xuan, Nguyen Thi1 |
| Instituciones: | 1University of Science and Technology, Vietnam Academy of Science and Technology, Hanói. Vietnam 2Institute of Genome Research, Vietnam Academy of Science and Technology, Hanói. Vietnam 3University of Science and Technology of Hanoi, Vietnam Academy of Science and Technology, Hanói. Vietnam 4108 Military Central Hospital, Hanói. Vietnam 5Vietnam Military Medical University, Department of Pathophysiology, Ha Dong, Hanói. Vietnam |
| Año: | 2022 |
| Volumen: | 45 |
| Número: | 4 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Analítico, descriptivo |
| Resumen en inglés | Psoriasis is a common chronic, immune-mediated inflammatory disease of the skin. PSORS1C3 is a non-protein coding gene, of which the RNA transcript is found in psoriatic patients. CARD14 is mainly expressed in epidermal keratinocytes. TLR4 is a transmembrane protein to recognize microbial antigens. Our study aimed to assess the relationship among PSORS1C3, CARD14 and TLR4 polymorphisms, inflammatory expression and psoriasis susceptibility. To the end, 71 patients with psoriasis and 46 healthy individuals with the well-characterized clinical profiles were enrolled. Gene polymorphisms were determined by Sanger DNA sequencing and secretion of cytokines by ELISA. As a result, genetic analysis of PSORS1C3 gene identified nine SNPs and three haplotype blocks. Sequencing of the CARD14 gene determined eight SNPs and one haplotype block. Sequencing of TLR4 gene identified nine SNPs, in which a SNP rs1018673641 was found to exert deleterious effect. The linkage disequilibrium analysis showed that seven variants in PSORS1C3 gene and three SNPs in CARD14 gene were in tightly linked. More importantly, a significant association between IL-6 level and rs1018673641 AT genotype in TLR4 gene was detected in psoriatic patients. In conclusion, the PSORS1C3, CARD14 and TLR4 polymorphisms and haplotypes may be correlated with risk of suffering psoriasis and the IL-6-mediated chronic inflammation in psoriasis could be partially regulated by the TLR4 functional variant |
| Disciplinas: | Biología, Medicina |
| Palabras clave: | Genética, Dermatología, Polimorfismo genético, Genotipos, Haplotipos, Psoriasis |
| Keyword: | Genetics, Dermatology, Genetic polymorphism, Genotypes, Haplotypes, Psoriasis |
| Texte intégral: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
