| Revista: | Gaceta médica de Caracas |
| Base de datos: | |
| Número de sistema: | 000595979 |
| ISSN: | 2739-0012 |
| Autores: | Merdekawati, Fusvita1 Manurung, Jesicca Elisabeth1 Nurhayati, Betty1 |
| Instituciones: | 1Politeknik Kesehatan Kemenkes Bandung, Bandung, Indonesia, |
| Año: | 2023 |
| Volumen: | 131 |
| Número: | 4 |
| País: | Venezuela |
| Idioma: | Español |
| Resumen en español | Introduction: The widely used method for transformation was the heat shock method, which was influenced by the time and temperature of heat shock treatment. However, there was a lack of research on the optimal temperature for pGEM-T plasmidtransformation in Escherichia coli JM109. This study uses the heat shock method to determine the optimum temperature and time for pGEM-T transformation in Escherichia coli JM109. |
| Palabras clave: | Recombinant, technology, transformation, Escherichia coli JM109, imageJ |
| Texto completo: | Texto completo (Ver PDF) Texto completo (Ver HTML) |
