The use of rice straw broth as an appropriate medium to isolate purple nonsulfur bacteria from paddy fields
Título del documento: The use of rice straw broth as an appropriate medium to isolate purple nonsulfur bacteria from paddy fields
Revue: Electronic journal of biotechnology
Base de datos: PERIÓDICA
Número de sistema: 000357876
ISSN: 0717-3458
Autores: 1
1
2
2
Instituciones: 1Prince of Songkla University, Faculty of Science, Hat-Yai, Songkhla. Tailandia
2Okayama University, The Graduate School of Natural Science and Technology, Okayama. Japón
Año:
Volumen: 15
Número: 6
País: Chile
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés The aims were to explore an appropriate isolating medium for obtaining purple nonsulfur bacteria (PNSB) for use as biofertilizers in saline paddy fields and to obtain pure cultures. We therefore chose a defined isolating medium containing 0.25% NaCl, (Glutamate-Acetate broth, GA) and a rice straw broth to compare them for numbers of PNSB obtained, time to obtain pure cultures, diversity and costs. A total of 30 water and 30 sediment samples were collected from saline paddy fields in southern Thailand and used to isolate PNSB in both the isolating media. Based on 60 samples and a period of 13 days incubation under anaerobic light conditions, a greater number of samples produced PNSB growth in GA broth after only day 3; however, after that the rice straw broth provided about a 2 fold increase in the number of samples that produced PNSB growth. Colonies isolated from GA broth required a significantly higher number of repeated streaking to obtain a pure culture (average 3.5) than those from rice straw broth (average 2.7) and the latter medium also produced significantly (P < 0.05) more isolates per sample. Sixty samples of water and sediment, from rice paddies with salinity (average, 3.43 ± 0.67 mS/cm) and slight acidity (average, pH 5.84 ± 0.42) provided 62 PNSB isolates by GA broth and 210 isolates by rice straw broth, and rice straw broth also produced a greater prevalence of PNSB. Estimates of the costs based on current prices of media, Gas Pak and electricity to obtain PNSB with the use of GA broth was roughly 6 times higher than for the rice straw broth
Disciplinas: Biología,
Agrociencias
Palabras clave: Fertilización,
Biotecnología,
Cultivo de bacterias,
Medios de cultivo,
Bacterias púrpura,
Arrozales,
Aislamiento bacteriano,
Biofertilizantes
Keyword: Biology,
Agricultural sciences,
Fertilization,
Biotechnology,
Bacterial culture,
Culture media,
Purple bacteria,
Paddy fields,
Bacterial isolation,
Biofertilizers
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