| Revue: | Electronic journal of biotechnology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000359303 |
| ISSN: | 0717-3458 |
| Autores: | Sequeida, Alvaro1 Tapia, Eduardo1 Ortega, Marcelo2 Zamora, Pablo3 Castro, Alvaro2 Montes, Christian4 Zúñiga, Gustavo E5 Prieto, Humberto4 |
| Instituciones: | 1Universidad Técnica Federico Santa María, Programa Doctorado en Biotecnología, Valparaíso. Chile 2Universidad de Santiago de Chile, Programa Doctorado en Biotecnología, Santiago de Chile. Chile 3University of California, Plant Sciences Department, Davis, California. Estados Unidos de América 4Instituto de Investigaciones Agropecuarias, Centro Regional de Investigación La Platina, Santiago de Chile. Chile 5Universidad de Santiago de Chile, Facultad de Química y Biología, Santiago de Chile. Chile |
| Año: | 2012 |
| Volumen: | 15 |
| Número: | 4 |
| País: | Chile |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | Deschampsia antarctica (DA), the only species in the Gramineae family endemic to the Antarctic territory, is characterized by a combination of high levels of free endogenous phenylpropanoid compounds under normal in situ and in vitro growth conditions. In this article, we describe the design and use of a specific temporary immersion photobioreactor to produce both increased DA biomass and secondary metabolite accumulation by UV-B elicitation during cultivation. Three min-long immersions in an induction medium applied every 4 hrs at 14ºC ± 1 and 20/4 hrs light/darkness photoperiod increased DA biomass production over previous in vitro reports. Biomass duplication was obtained at day 10.7 of culturing, and maximum total phenolics and antioxidant activity were observed after 14 day of culturing. The addition of UV-B radiation pulses for 0.5 hrs at 6 hrs intervals increased total phenolics and antioxidant activity more than 3- and 1.5- fold, respectively, compared to controls with no UV-B. Significant accumulation of scopoletin, chlorogenic acid, gallic acid and rutin was found in these plantlets. This is the first bioreactor designed to optimize biomass and phenylpropanoid production in DA |
| Disciplinas: | Biología, Química |
| Palabras clave: | Biotecnología, Bioproducción, Biorreactores, Fotobiorreactores, Luz ultravioleta, Compuestos fenólicos, Deschampsia antarctica, Gramíneas |
| Keyword: | Biology, Chemistry, Biotechnology, Bioproduction, Bioreactors, Photobioreactors, Ultraviolet light, Phenolic compounds, Deschampsia antarctica, Gramineae |
| Texte intégral: | Texto completo (Ver HTML) |
