| Revue: | Electronic journal of biotechnology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000267078 |
| ISSN: | 0717-3458 |
| Autores: | Lawrence, Paulraj Kirubakaran1 Nirmala, Jayaveeramuthu Koundal, Kripa Ram2 |
| Instituciones: | 1Texas Tech University, School of Medicine, Lubbock, Texas. Estados Unidos de América 2Indian Council of Agricultural Research, Indian Agricultural Research Institute, Nueva Delhi, Delhi. India |
| Año: | 2001 |
| Periodo: | Abr |
| Volumen: | 4 |
| Número: | 1 |
| País: | Chile |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental |
| Resumen en inglés | A protease inhibitor gene has been isolated from native cowpea (Vigna unguiculata L.) cv. 130 from l-ZAP II genomic library. The nucleotide sequence of this genomic clone shared 86% homology with a cowpea trypsin inhibitor f IV mRNA, and 81% with Bowman-Birk protease inhibitor genes of soybean. The isolated gene had TATA and CAT signals in the 5' upstream region. The longest open reading frame had 504 bases, encoding 167 amino acids from the predicted coding region. The active serine centers of this protein was found between 105-158 amino acid residues, with a 69 amino acids long signal peptide in its N terminal |
| Disciplinas: | Agrociencias |
| Palabras clave: | Leguminosas, Biotecnología, Genética, Caupí, Germoplasma, Vigna unguiculata, Nucleótidos, Clones, Tripsina |
| Keyword: | Agricultural sciences, Legumes, Cowpea, Germplasm, Vigna unguiculata, Nucleotides, Clones, Trypsin, Genetics |
| Texte intégral: | Texto completo (Ver HTML) |
