| Revue: | Electronic journal of biotechnology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000358917 |
| ISSN: | 0717-3458 |
| Autores: | Zhang, Yuejin1 Qin, Yali1 Guo, Lijun1 Zhou, Zili1 Liang, Zongsuo1 Zhang, Chenlu1 Guo, Hongbo1 |
| Instituciones: | 1Northwest A & F University, College of Life Sciences, Yangling, Shaanxi. China |
| Año: | 2012 |
| Volumen: | 15 |
| Número: | 5 |
| País: | Chile |
| Idioma: | Inglés |
| Tipo de documento: | Nota breve o noticia |
| Enfoque: | Experimental, analítico |
| Resumen en inglés | The dried sclerotium of medicinal fungus Polyporus umbellatus (Pers.) Fries has many pharmacological functions such as diuretic and anticancer activity, in which high-content polysaccharides may play an important role. However, RNA isolation is difficult in filamentous fungi and lacking in P. umbellatus. Results: Five methods for RNA extraction from five strains collected from four provinces were assessed for their ability to recover a high-quality RNA applicable for sequence-related amplification polymorphism (SRAP) PCR and GDP-D-mannose pyrophosphorylase (GMP) gene expression profiles. Both A260/A280 and A260/A230 ratios of the best Trizol Plus + RNAiso-mate for Plant Tissue method are around 2 with a yield of 1122.00 ± 0.21 ng μl-1. The Trizol method also showed good quality with the yield 469.60 ng μl-1. The SRAP PCR amplified clear and polymorphic bands in all five cDNA samples transcribed from RNA by using primer Me4-Em4. GMP gene fragment (1251 bp) was successfully amplified by RT-PCR, suggesting the integrity of isolated RNA. Conclusion: All these results showed that the total RNA isolated by this protocol is of sufficient quality for subsequent molecular applications |
| Disciplinas: | Biología |
| Palabras clave: | Genética, Hongos, Polyporus umbellatus, Especies medicinales, ARN, Biotecnología |
| Keyword: | Biology, Fungi, Genetics, Polyporus umbellatus, Medicinal species, RNA, Biotechnology |
| Texte intégral: | Texto completo (Ver HTML) |
