| Revue: | Electronic journal of biotechnology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000336243 |
| ISSN: | 0717-3458 |
| Autores: | Pavlov, Maria Soledad1 Eissler, Yoanna1 Espinoza, Juan Carlos1 Garland, David E1 Kuznar, Juan1 |
| Instituciones: | 1Universidad de Valparaíso, Facultad de Ciencias, Valparaíso. Chile |
| Año: | 2011 |
| Periodo: | Ene |
| Volumen: | 14 |
| Número: | 1 |
| País: | Chile |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental |
| Resumen en inglés | A method for counting Infectious pancreatic necrosis virus (IPNV) through epifluorescence microscopy was analyzed in detail. Image processing and statistic considerations are included. The particle size of viruses was compared in different experimental conditions such as the staining of the virus with SYBR-Green I or with antibodies for specific fluorescence labeling of viral proteins. The type of surface used as mounting support was assayed as well. The results indicated that the most suitable method involves the mounting of the viral-containing suspension on a membrane filter followed by the staining with a monoclonal antibody specific for a viral protein combined with a FITC (fluorescein isothiocyanate)-conjugated secondary antibody |
| Disciplinas: | Biología, Medicina veterinaria y zootecnia |
| Palabras clave: | Peces, Virus, Biotecnología, Virus de la infección necrosante pancreática, Microscopía de fluorescencia, Inmunofluorescencia |
| Keyword: | Biology, Veterinary medicine and animal husbandry, Fish, Virus, Biotechnology, Infectious pancreatic necrosis virus, Fluorescence microscopy, Immunofluorescence |
| Texte intégral: | Texto completo (Ver PDF) |
