| Revue: | Electronic journal of biotechnology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000266056 |
| ISSN: | 0717-3458 |
| Autores: | Afaq, Sarah1 Iqbal, Jawaid2 |
| Instituciones: | 1Aligarh Muslim University, Faculty of Life Sciences, Aligarh, Uttar Pradesh. India 2Aligarh Muslim University, Faculty of Life Sciences, Aligarh, Uttar Pradesh. India |
| Año: | 2001 |
| Periodo: | Dic |
| Volumen: | 4 |
| Número: | 3 |
| Paginación: | 1-2 |
| País: | Chile |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, analítico |
| Resumen en inglés | A method for immobilization of papain has been selected based on the interaction between its histidine, cysteine and tryptophan residues with the immobilized metal ion (IMI) carrier for maximum binding on a small volume of the carrier. The immobilized papain retained high activity has improved thermal stability and the carrier could be recovered from the spent bound enzyme, to be reused. Reimmobilization of papain on the regenerated matrix was equally effective with the retention of maximum enzyme activity |
| Disciplinas: | Química |
| Palabras clave: | Biotecnología, Inmunología, Papaína, Iones metálicos, Estabilidad térmica |
| Keyword: | Chemistry, Papain, Metal ions, Thermal stability, Immunology |
| Texte intégral: | Texto completo (Ver HTML) |
