| Revue: | Electronic journal of biotechnology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000358108 |
| ISSN: | 0717-3458 |
| Autores: | Lima, Alita Moura de1 Salem, Julia Ignez1 Souza, Joao Vicente Braga de1 Cortez, Ana Claudia Alves1 Carvalho, Clarice Maia2 Chaves, Francisco Celio Maia3 Veiga-Junior, Valdir Florencio da4 |
| Instituciones: | 1Instituto Nacional de Pesquisas da Amazonia, Coordenacao de Pesquisas em Ciencias da Saude, Manaus, Amazonas. Brasil 2Centro de Biotecnologia da Amazonia, Departamento de Microbiologia, Manaus, Amazonas. Brasil 3Empresa Brasileira de Pesquisa Agropecuaria, Departamento de Plantas Medicinais, Manaus, Amazonas. Brasil 4Universidade Federal do Amazonas, Departamento de Quimica, Manaus, Amazonas. Brasil |
| Año: | 2011 |
| Volumen: | 14 |
| Número: | 4 |
| País: | Chile |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | Substances that inhibit the growth of Mycobacterium tuberculosis could potentially be used as antibiotics. These substances could also be added to test culture media to improve the speed of tuberculosis diagnosis. The aim of this work was to investigate the influence of culture filtrates of endophytic fungi isolated from P. aduncum L. on the growth of M. tuberculosis. To achieve this objective, the following methodology was used: a) endophytic fungi were isolated from the leaves and stems of P. aduncum L.; b) the isolated fungi were submitted to submerged bioprocessing; c) culture filtrates from the bioprocess were assayed to evaluate their effect on the growth of M. tuberculosis. We isolated 315 fungal types, which represented 85 morphologies, from different parts of P. aduncum L. The bioassays were performed on 82 culture filtrates and 6 plant extracts and resulted in the detection of 1 culture filtrate that stimulated the growth of M. tuberculosis and 15 that inhibited microbial growth. None of the phytochemical extracts had an effect on the growth of M. tuberculosis. In conclusion, we observed that the endophytic fungi isolated from P. aduncum L. (Piperaceae) produced extracellular metabolites (present in the culture filtrate) that affect the growth of M. tuberculosis. These compounds have the potential to be used as antimicrobials or in the diagnosis of tuberculosis |
| Disciplinas: | Biología |
| Palabras clave: | Biotecnología, Farmacología, Hongos endofíticos, Inhibición del crecimiento, Mycobacterium tuberculosis |
| Keyword: | Biology, Biotechnology, Pharmacology, Endophytic fungi, Growth inhibition, Mycobacterium tuberculosis |
| Texte intégral: | Texto completo (Ver HTML) |
